PMID- 23776031
OWN - NLM
STAT- MEDLINE
DCOM- 20140117
LR  - 20130618
IS  - 1612-1880 (Electronic)
IS  - 1612-1872 (Linking)
VI  - 10
IP  - 6
DP  - 2013 Jun
TI  - Spectroscopic and biological studies of phenanthroline compounds: selective 
      recognition of gene-promoter G-quadruplex DNAs preferred over duplex DNA.
PG  - 1154-64
LID - 10.1002/cbdv.201200341 [doi]
AB  - G-Quadruplex DNA formed in the promoter regions of proto-oncogenes would block 
      the transcription process and eventually suppress the development of tumors, so 
      compounds that stabilize G-quadruplex DNA are potential antitumor drugs. This 
      article describes the interactions of three phenanthroline compounds, a-c, with 
      proto-oncogene c-kit2 and c-myc G-quadruplex DNAs by means of polymerase chain 
      reaction (PCR) stop assay, fluorescence resonance energy transfer melting (FRET 
      melting) assay, fluorescence indicator displacement (FID) assay, UV/VIS 
      absorption, fluorescence, and circular dichroism (CD) spectroscopies. All three 
      compounds displayed selectivity for the G-quadruplex over duplex, with binding 
      constants (Ka) for the both quadruplexes varying from 0.49x10(6) to 3.32x10(6) M(-)(1) 
      (4.1- to 33.2-fold specificity). Compounds a, b, and c were potential stabilizers 
      for the c-kit2 G-quadruplex with the melting-temperature increase (DeltaTm) values of 
      12-15 degrees . CD Spectra indicated that a-c disrupted the structure of c-kit2 
      G-quadruplex, whereas no significant change was observed for c-myc G-quadruplex.
CI  - Copyright (c) 2013 Verlag Helvetica Chimica Acta AG, Zurich.
FAU - Wang, Lihua
AU  - Wang L
AD  - Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of 
      Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR 
      China.
FAU - Wei, Chunying
AU  - Wei C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Chem Biodivers
JT  - Chemistry & biodiversity
JID - 101197449
RN  - 0 (Phenanthrolines)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Circular Dichroism
MH  - DNA/*chemistry/metabolism
MH  - Fluorescence Resonance Energy Transfer
MH  - *G-Quadruplexes
MH  - Phenanthrolines/*chemistry/metabolism
MH  - Proto-Oncogene Proteins c-kit/genetics
MH  - Proto-Oncogene Proteins c-myc/genetics
MH  - Spectrophotometry, Ultraviolet
MH  - Transition Temperature
EDAT- 2013/06/19 06:00
MHDA- 2014/01/18 06:00
CRDT- 2013/06/19 06:00
PHST- 2012/10/05 00:00 [received]
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/18 06:00 [medline]
AID - 10.1002/cbdv.201200341 [doi]
PST - ppublish
SO  - Chem Biodivers. 2013 Jun;10(6):1154-64. doi: 10.1002/cbdv.201200341.