PMID- 23776529
OWN - NLM
STAT- MEDLINE
DCOM- 20140116
LR  - 20220311
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 6
DP  - 2013
TI  - Hypertrophy dependent doubling of L-cells in Roux-en-Y gastric bypass operated 
      rats.
PG  - e65696
LID - 10.1371/journal.pone.0065696 [doi]
LID - e65696
AB  - BACKGROUND AND AIMS: Roux-en-Y gastric bypass (RYGB) leads to a rapid remission 
      of type 2 diabetes mellitus (T2DM), but the underlying mode of action remains 
      incompletely understood. L-cell derived gut hormones such as glucagon-like 
      peptide-1 (GLP-1) and peptide YY (PYY) are thought to play a central role in the 
      anti-diabetic effects of RYGB; therefore, an improved understanding of intestinal 
      endocrine L-cell adaptability is considered pivotal. METHODS: The full 
      rostrocaudal extension of the gut was analyzed in rats after RYGB and in 
      sham-operated controls ad libitum fed or food restricted to match the body weight 
      of RYGB rats. Total number of L-cells, as well as regional numbers, densities and 
      mucosa volumes were quantified using stereological methods. Preproglucagon and 
      PYY mRNA transcripts were quantified by qPCR to reflect the total and relative 
      hormone production capacity of the L-cells. RESULTS: RYGB surgery induced 
      hypertrophy of the gut mucosa in the food exposed regions of the small intestine 
      coupled with a doubling in the total number of L-cells. No changes in L-cell 
      density were observed in any region regardless of surgery or food restriction. 
      The total gene expression capacity of the entire gut revealed a near 200% 
      increase in both PYY and preproglucagon mRNA levels in RYGB rats associated with 
      both increased L-cell number as well as region-specific increased transcription 
      per cell. CONCLUSIONS: Collectively, these findings indicate that RYGB in rats is 
      associated with gut hypertrophy, an increase in L-cell number, but not density, 
      and increased PYY and preproglucagon gene expression. This could explain the 
      enhanced gut hormone dynamics seen after RYGB.
FAU - Hansen, Carl Frederik
AU  - Hansen CF
AD  - Department of Histology, Gubra, Horsholm, Denmark ; Department of Human 
      Nutrition, University of Copenhagen, Frederiksberg, Denmark.
FAU - Bueter, Marco
AU  - Bueter M
FAU - Theis, Nadine
AU  - Theis N
FAU - Lutz, Thomas
AU  - Lutz T
FAU - Paulsen, Sarah
AU  - Paulsen S
FAU - Dalboge, Louise S
AU  - Dalboge LS
FAU - Vrang, Niels
AU  - Vrang N
FAU - Jelsing, Jacob
AU  - Jelsing J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130611
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 106388-42-5 (Peptide YY)
RN  - 55963-74-1 (Proglucagon)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Animals
MH  - Enteroendocrine Cells/*cytology/metabolism
MH  - Gastric Bypass/*adverse effects
MH  - Glucagon-Like Peptide 1/genetics/metabolism
MH  - Hypertrophy/etiology/*physiopathology
MH  - Male
MH  - Mucous Membrane/metabolism
MH  - Peptide YY/genetics/metabolism
MH  - Proglucagon/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
PMC - PMC3679162
COIS- Competing Interests: The following authors are currently employed by the 
      commercial company Gubra Aps: Niels Vrang, Louise Schjellerup Dalboge, Sarah 
      Paulsen, Jacob Jelsing and Carl Frederik Hansen. However, this does not alter the 
      authors' adherence to all the PLOS ONE policies on sharing data and materials.
EDAT- 2013/06/19 06:00
MHDA- 2014/01/17 06:00
PMCR- 2013/06/11
CRDT- 2013/06/19 06:00
PHST- 2012/11/09 00:00 [received]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/17 06:00 [medline]
PHST- 2013/06/11 00:00 [pmc-release]
AID - PONE-D-12-34899 [pii]
AID - 10.1371/journal.pone.0065696 [doi]
PST - epublish
SO  - PLoS One. 2013 Jun 11;8(6):e65696. doi: 10.1371/journal.pone.0065696. Print 2013.