PMID- 23776540
OWN - NLM
STAT- MEDLINE
DCOM- 20140116
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 6
DP  - 2013
TI  - Identification of cancer stem-like side population cells in purified primary 
      cultured human laryngeal squamous cell carcinoma epithelia.
PG  - e65750
LID - 10.1371/journal.pone.0065750 [doi]
LID - e65750
AB  - Cancer stem-like side population (SP) cells have been identified in many solid 
      tumors; however, most of these investigations are performed using established 
      cancer cell lines. Cancer cells in tumor tissue containing fibroblasts and many 
      other types of cells are much more complex than any cancer cell line. Although SP 
      cells were identified in the laryngeal squamous cell carcinoma (LSCC) cell line 
      Hep-2 in our pilot study, it is unknown whether the LSCC tissue contains SP 
      cells. In this study, LSCC cells (LSCCs) were primary cultured and purified from 
      a surgically resected LSCC specimen derived from a well-differentiated epiglottic 
      neoplasm of a Chinese male. This was followed by the verification of 
      epithelium-specific characteristics, such as ultrastructure and biomarkers. A 
      distinct SP subpopulation (4.45+/-1.07%) was isolated by Hoechst 33342 efflux 
      analysis from cultured LSCCs by using a flow cytometer. Cancer stem cell 
      (CSC)-associated assays, including expression of self-renewal and CSC marker 
      genes, proliferation, differentiation, spheroid formation, chemotherapy 
      resistance, and tumorigenicity were then conducted between SP and non-SP (NSP) 
      LSCCs. In vitro and in vivo assays revealed that SP cells manifested preferential 
      expression of self-renewal and CSC marker genes, higher capacity for 
      proliferation, differentiation, and spheroid formation; enhanced resistance to 
      chemotherapy; and greater xenograft tumorigenicity in immunodeficient mice 
      compared with NSP cells. These findings suggest that the primary cultured and 
      purified LSCCs contain cancer stem-like SP cells, which may serve as a valuable 
      model for CSC research in LSCC.
FAU - Wu, Chun-Ping
AU  - Wu CP
AD  - Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose and Throat 
      Hospital, Fudan University, Shanghai, People's Republic of China.
FAU - Zhou, Liang
AU  - Zhou L
FAU - Xie, Ming
AU  - Xie M
FAU - Du, Huai-Dong
AU  - Du HD
FAU - Tian, Jie
AU  - Tian J
FAU - Sun, Shan
AU  - Sun S
FAU - Li, Jin-Yan
AU  - Li JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130611
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Aged
MH  - Animals
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*pathology/ultrastructure
MH  - Cell Differentiation/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Survival/physiology
MH  - Flow Cytometry
MH  - Head and Neck Neoplasms/*pathology/ultrastructure
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Microscopy, Electron, Transmission
MH  - Neoplastic Stem Cells/*cytology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Side-Population Cells/*cytology/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Tumor Cells, Cultured
PMC - PMC3679141
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/06/19 06:00
MHDA- 2014/01/17 06:00
PMCR- 2013/06/11
CRDT- 2013/06/19 06:00
PHST- 2012/11/26 00:00 [received]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/17 06:00 [medline]
PHST- 2013/06/11 00:00 [pmc-release]
AID - PONE-D-12-37199 [pii]
AID - 10.1371/journal.pone.0065750 [doi]
PST - epublish
SO  - PLoS One. 2013 Jun 11;8(6):e65750. doi: 10.1371/journal.pone.0065750. Print 2013.