PMID- 23776573
OWN - NLM
STAT- MEDLINE
DCOM- 20140116
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 6
DP  - 2013
TI  - Enhancing proprioceptive input to motoneurons differentially affects expression 
      of neurotrophin 3 and brain-derived neurotrophic factor in rat hoffmann-reflex 
      circuitry.
PG  - e65937
LID - 10.1371/journal.pone.0065937 [doi]
LID - e65937
AB  - The importance of neurotrophin 3 (NT-3) for motor control prompted us to ask the 
      question whether direct electrical stimulation of low-threshold muscle afferents, 
      strengthening the proprioceptive signaling, could effectively increase the 
      endogenous pool of this neurotrophin and its receptor TrkC in the Hoffmann-reflex 
      (H-reflex) circuitry. The effects were compared with those of brain-derived 
      neurotrophic factor (BDNF) and its TrkB receptor. Continuous bursts of stimuli 
      were delivered unilaterally for seven days, 80 min daily, by means of a 
      cuff-electrode implanted over the tibial nerve in awake rats. The H-reflex was 
      recorded in the soleus muscle to control the strength of stimulation. Stimulation 
      aimed at activation of Ia fibers produced a strong increase of NT-3 protein, 
      measured with ELISA, in the lumbar L3-6 segments of the spinal cord and in the 
      soleus muscle. This stimulation exerted much weaker effect on BDNF protein level 
      which slightly increased only in L3-6 segments of the spinal cord. Increased 
      protein level of NT-3 and BDNF corresponded to the changes of NT-3 mRNA and BDNF 
      mRNA expression in L3-6 segments but not in the soleus muscle. We disclosed 
      tissue-specificity of TrkC mRNA and TrkB mRNA responses. In the spinal cord TrkC 
      and TrkB transcripts tended to decrease, whereas in the soleus muscle TrkB mRNA 
      decreased and TrkC mRNA expression strongly increased, suggesting that 
      stimulation of Ia fibers leads to sensitization of the soleus muscle to NT-3 
      signaling. The possibility of increasing NT-3/TrkC signaling in the neuromuscular 
      system, with minor effects on BDNF/TrkB signaling, by means of low-threshold 
      electrical stimulation of peripheral nerves, which in humans might be applied in 
      non-invasive way, offers an attractive therapeutic tool.
FAU - Gajewska-Wozniak, Olga
AU  - Gajewska-Wozniak O
AD  - Department of Neurophysiology, Nencki Institute of Experimental Biology, Warsaw, 
      Poland.
FAU - Skup, Malgorzata
AU  - Skup M
FAU - Kasicki, Stefan
AU  - Kasicki S
FAU - Ziemlinska, Ewelina
AU  - Ziemlinska E
FAU - Czarkowska-Bauch, Julita
AU  - Czarkowska-Bauch J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130611
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
EIN - PLoS One. 2014;9(1). doi:10.1371/annotation/196dc3ba-c963-46ee-a41d-2cb862ef2736
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Electrophysiology
MH  - H-Reflex/genetics/*physiology
MH  - Male
MH  - Motor Neurons/*metabolism
MH  - Neurotrophin 3/genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/metabolism
MH  - Receptor, trkC/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3679030
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/06/19 06:00
MHDA- 2014/01/17 06:00
PMCR- 2013/06/11
CRDT- 2013/06/19 06:00
PHST- 2013/02/04 00:00 [received]
PHST- 2013/04/30 00:00 [accepted]
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/17 06:00 [medline]
PHST- 2013/06/11 00:00 [pmc-release]
AID - PONE-D-13-05151 [pii]
AID - 10.1371/journal.pone.0065937 [doi]
PST - epublish
SO  - PLoS One. 2013 Jun 11;8(6):e65937. doi: 10.1371/journal.pone.0065937. Print 2013.