PMID- 23777925
OWN - NLM
STAT- MEDLINE
DCOM- 20140718
LR  - 20131011
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 74
IP  - 11
DP  - 2013 Nov
TI  - The outcome of the endothelial precursor cell crossmatch test in lymphocyte 
      crossmatch positive and negative patients evaluated for living donor kidney 
      transplantation.
PG  - 1437-44
LID - S0198-8859(13)00162-6 [pii]
LID - 10.1016/j.humimm.2013.06.005 [doi]
AB  - The presence of human leukocyte antigen (HLA) and non-HLA antibodies (Abs) in 
      kidney transplant recipients is associated with graft rejections. This study 
      reports the results of an endothelial precursor cell crossmatch (EPCXM) test for 
      detection of non-HLA Abs and its correlation to lymphocyte crossmatch (LXM) test 
      results, the degree and type of sensitization, and transplantation (Tx) outcome 
      in patients evaluated for living donor (LD) kidney transplantation (KTx). 
      Patients were tested before any pre-transplantation (pre-Tx) treatment and at Tx. 
      Pre-Tx treatments included B cell depletion and Ab removal. Patient records were 
      reviewed for assessment of renal graft function, results of biopsies, and 
      identification of complications affecting the graft. Pre-Tx sera from 32% of the 
      LD patients had IgG and/or IgM-binding donor EPCs. Twenty-five percent of the 
      patients were EPCXM IgM+. Of the patients with negative LXM tests, 25% had EPC 
      Abs mainly of IgM class not reactive with HLA. There was no difference in 
      rejection frequency or serum creatinine levels between the EPCXM+ and EPCXM- 
      groups. The pre-Tx EPCXM+ group had significantly more patients with delayed 
      graft function. Prospective studies with appropriate control groups are needed to 
      establish whether pre-treatments aiming at removing anti-endothelial cell 
      antibodies, as detected by the EPCXM pre-Tx, have a beneficial effect on 
      short-term and long-term graft survival.
CI  - Copyright (c) 2013 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Alheim, Mats
AU  - Alheim M
AD  - Division of Clinical Immunology and Transfusion Medicine, Karolinska Institute, 
      Karolinska University Hospital in Huddinge, Stockholm, Sweden.
FAU - AlMahri, Ayeda
AU  - AlMahri A
FAU - Nilsson, Jakob
AU  - Nilsson J
FAU - Tyden, Gunnar
AU  - Tyden G
FAU - Holgersson, Jan
AU  - Holgersson J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130615
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Delayed Graft Function/*diagnosis/epidemiology/etiology
MH  - Endothelial Cells/immunology/*metabolism
MH  - Female
MH  - Graft Rejection/*diagnosis/epidemiology/etiology
MH  - Histocompatibility Testing/statistics & numerical data
MH  - Humans
MH  - Isoantibodies/blood
MH  - *Kidney Transplantation
MH  - Living Donors
MH  - Lymphocytes/immunology
MH  - Male
MH  - Middle Aged
MH  - Postoperative Complications/*diagnosis/epidemiology
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Stem Cells/immunology/*metabolism
MH  - Treatment Outcome
MH  - Young Adult
EDAT- 2013/06/20 06:00
MHDA- 2014/07/19 06:00
CRDT- 2013/06/20 06:00
PHST- 2012/10/28 00:00 [received]
PHST- 2013/03/27 00:00 [revised]
PHST- 2013/06/07 00:00 [accepted]
PHST- 2013/06/20 06:00 [entrez]
PHST- 2013/06/20 06:00 [pubmed]
PHST- 2014/07/19 06:00 [medline]
AID - S0198-8859(13)00162-6 [pii]
AID - 10.1016/j.humimm.2013.06.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2013 Nov;74(11):1437-44. doi: 10.1016/j.humimm.2013.06.005. Epub 
      2013 Jun 15.