PMID- 23778529
OWN - NLM
STAT- MEDLINE
DCOM- 20130920
LR  - 20240322
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Print)
IS  - 0007-0920 (Linking)
VI  - 109
IP  - 1
DP  - 2013 Jul 9
TI  - Biological evidence for a causal role of HPV16 in a small fraction of laryngeal 
      squamous cell carcinoma.
PG  - 172-83
LID - 10.1038/bjc.2013.296 [doi]
AB  - BACKGROUND: Human papillomavirus (HPV) is a causal factor in virtually all 
      cervical and a subset of oropharyngeal squamous cell carcinoma (OP-SCC), whereas 
      its role in laryngeal squamous cell carcinoma (L-SCC) is unclear. METHODS: 
      Formalin-fixed paraffin-embedded (N=154) and deep-frozen tissues (N=55) of 102 
      L-SCC patients were analysed for the presence of 51 mucosal HPV types. HPV 
      DNA-positive (HPV DNA+) cases were analysed for E6*I mRNA transcripts of all high 
      risk (HR)/probably/possibly (p)HR-HPV identified, and for HPV type 16 (HPV16) 
      viral load. Expression of p16(INK4a), pRb, cyclin D1 and p53 was analysed by 
      immunohistochemistry. RESULTS: Ninety-two patients were valid in DNA analysis, of 
      which 32 (35%) had at least one HPV DNA+ sample. Among the 29 single infections, 
      22 (76%) were HPV16, 2 (7%) HPV56 and 1 each (4%) HPV45, HPV53, HPV70, HPV11 and 
      HPV42. Three cases harboured HPV16 with HPV33 (twice) or HPV45. Only 32% of HPV 
      DNA+ findings were reproducible. Among HPV16 DNA+ L-SCC, 2 out of 23 (9%) had 
      high viral loads, 5 out of 25 (21%) expressed E6*I mRNA and 3 out of 21 (14%) 
      showed high p16(INK4a) and low pRb expression (all three HPV16 RNA-positive), 
      immunohistochemical marker combination not identified in any other HPV DNA+ or 
      HPV DNA-negative (HPV DNA-) L-SCC, respectively. CONCLUSION: HPV type 16 has a 
      causative role in a small subgroup of L-SCC (<5% in this German hospital series).
FAU - Halec, G
AU  - Halec G
AD  - Division of Genome Modifications and Carcinogenesis, Infections and Cancer 
      Program, German Cancer Research Center, Im Neuenheimer Feld 242, 69120 
      Heidelberg, Germany. g.halec@dkfz.de
FAU - Holzinger, D
AU  - Holzinger D
FAU - Schmitt, M
AU  - Schmitt M
FAU - Flechtenmacher, C
AU  - Flechtenmacher C
FAU - Dyckhoff, G
AU  - Dyckhoff G
FAU - Lloveras, B
AU  - Lloveras B
FAU - Hofler, D
AU  - Hofler D
FAU - Bosch, F X
AU  - Bosch FX
FAU - Pawlita, M
AU  - Pawlita M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130618
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*virology
MH  - DNA, Viral/analysis/isolation & purification
MH  - Female
MH  - Genotype
MH  - Human papillomavirus 16/genetics/isolation & purification/*physiology
MH  - Humans
MH  - Laryngeal Neoplasms/*virology
MH  - Male
MH  - Middle Aged
MH  - Oropharyngeal Neoplasms/*virology
MH  - Papillomavirus Infections/virology
MH  - Viral Load
PMC - PMC3708587
EDAT- 2013/06/20 06:00
MHDA- 2013/09/21 06:00
PMCR- 2014/07/09
CRDT- 2013/06/20 06:00
PHST- 2013/03/11 00:00 [received]
PHST- 2013/05/16 00:00 [revised]
PHST- 2013/05/21 00:00 [accepted]
PHST- 2013/06/20 06:00 [entrez]
PHST- 2013/06/20 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
PHST- 2014/07/09 00:00 [pmc-release]
AID - bjc2013296 [pii]
AID - 10.1038/bjc.2013.296 [doi]
PST - ppublish
SO  - Br J Cancer. 2013 Jul 9;109(1):172-83. doi: 10.1038/bjc.2013.296. Epub 2013 Jun 
      18.