PMID- 23779096
OWN - NLM
STAT- MEDLINE
DCOM- 20140324
LR  - 20211021
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 30
IP  - 7
DP  - 2013 Jul
TI  - Activation of the LH receptor up regulates the type 2 adiponectin receptor in 
      human granulosa cells.
PG  - 963-8
LID - 10.1007/s10815-013-0012-3 [doi]
AB  - PURPOSE: Adiponectin is a predominantly adipocyte-derived hormone which 
      influences insulin sensitivity and energy homeostasis through at least two 
      receptors, AdipoR1 and AdipoR2. In animal models, adiponectin may regulate 
      ovarian steroidogenesis, folliculogenesis, and ovulation. The receptors AdipoR1 
      and AdipoR2 are present in the human ovary, but their regulation is unknown. In 
      these studies, we determined the effects of LH receptor activation on the 
      expression and function of the two adiponectin receptors in human granulosa 
      cells. METHODS: Granulosa cells were obtained at the time of oocyte retrieval in 
      women undergoing in vitro fertilization (IVF). Cells were isolated and cultured 
      for 48 h in DMEM/F12 medium with 5 % FBS and 50 ug/ml gentamicin. Medium was 
      changed to low serum for 12 h and cells were treated with hCG (100 ng/ml), 
      forskolin (30 muMol/L), or FSH (1 IU/ml) for 24 h for mRNA experiments. mRNA was 
      isolated and RT PCR was performed using Taqman assays and quantification with the 
      delta delta CT method. For immunocytochemistry, cells were grown on chamber 
      slides and treated with hCG for 1 to 24 h and fixed with acetone. ICC was 
      performed with polyclonal rabbit primary antibodies followed by alexa fluor goat 
      anti-rabbit antibody and imaging with a fluorescence microscope and Zeiss 
      software analysis. 3beta-hydroxysteroid dehydrogenase (3betaHSD) enzyme activity was 
      determined by measuring the progesterone produced when cells were provided with 
      an excess of 22-hydroxy-cholesterol as substrate following an incubation with hCG 
      (1 IU/ml) and/or adiponectin (10 ng/ml). Progesterone content in the media was 
      determined by ELISA. RESULTS: Messenger RNA for the two Adiponectin receptors is 
      differentially regulated by activation of LHR with hCG treatment. AdipoR2 was 
      increased nearly 4-fold (p < 0.05), whereas AdipoR1 expression was not changed by 
      hCG treatment. Treatment with either FSH or forskolin (an activator of cAMP) had 
      similar effects. Basal AdipoR2 protein was fairly low in granulosa cells in 
      culture however treatment of cells with hCG resulted in a discernible increase in 
      immunodetectable cytoplasmic protein as early as 6 h after treatment and was 
      maintained for at least 24 h. The number of cells positive for AdipoR2 at 6 h 
      increased from a basal of 20 % to almost 60 % (p < 0.05). Adiponectin treatment 
      of hCG-primed cells resulted in increased 3betaHSD activity by approximately 60 % 
      over hCG alone and more than 3-fold over basal levels. CONCLUSIONS: AdipoR2 is 
      regulated by the LH receptor function via a cAMP dependant mechanism. Increased 
      expression of adipoR2 prior to and following ovulation may contribute to enhanced 
      3betaHSD activity and increased progesterone secretion by the corpus luteum of the 
      ovary. Dysregulation of adiponectin that may occur with PCOS may impair normal 
      progesterone production.
FAU - Wickham, Edmond P 3rd
AU  - Wickham EP 3rd
AD  - Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA 
      23298, USA.
FAU - Tao, Tao
AU  - Tao T
FAU - Nestler, John E
AU  - Nestler JE
FAU - McGee, Elizabeth A
AU  - McGee EA
LA  - eng
GR  - R01 HD045700/HD/NICHD NIH HHS/United States
GR  - U54 HD034449/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20130619
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (ADIPOR1 protein, human)
RN  - 0 (ADIPOR2 protein, human)
RN  - 0 (Adiponectin)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Adiponectin)
RN  - 0 (Receptors, LH)
RN  - 1F7A44V6OU (Colforsin)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - Adiponectin/metabolism
MH  - Cells, Cultured
MH  - Chorionic Gonadotropin/metabolism
MH  - Colforsin/metabolism
MH  - Cyclic AMP/metabolism
MH  - Female
MH  - Fertilization in Vitro
MH  - Follicle Stimulating Hormone/metabolism
MH  - Granulosa Cells/*metabolism
MH  - Humans
MH  - Oocyte Retrieval
MH  - Progesterone/biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Adiponectin/*biosynthesis/genetics/*metabolism
MH  - Receptors, LH/*metabolism
MH  - Up-Regulation
PMC - PMC3725221
EDAT- 2013/06/20 06:00
MHDA- 2014/03/25 06:00
PMCR- 2013/06/19
CRDT- 2013/06/20 06:00
PHST- 2012/11/25 00:00 [received]
PHST- 2013/05/16 00:00 [accepted]
PHST- 2013/06/20 06:00 [entrez]
PHST- 2013/06/20 06:00 [pubmed]
PHST- 2014/03/25 06:00 [medline]
PHST- 2013/06/19 00:00 [pmc-release]
AID - 12 [pii]
AID - 10.1007/s10815-013-0012-3 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2013 Jul;30(7):963-8. doi: 10.1007/s10815-013-0012-3. Epub 
      2013 Jun 19.