PMID- 23780496
OWN - NLM
STAT- MEDLINE
DCOM- 20140117
LR  - 20191210
IS  - 1807-3107 (Electronic)
IS  - 1806-8324 (Linking)
VI  - 27
IP  - 4
DP  - 2013 Jul-Aug
TI  - Influence of substratum position and acquired pellicle on Candida albicans 
      biofilm.
PG  - 369-75
LID - S1806-83242013005000020 [pii]
LID - 10.1590/S1806-83242013005000020 [doi]
AB  - The purpose of this study was to evaluate the influence of the substratum 
      position and the saliva acquired pellicle (AP) on Candida albicans biofilm 
      development. Poly(methylmethacrylate) (PMMA) disks were fabricated and randomly 
      allocated to experimental groups: HNP (disks placed in a horizontal position and 
      uncoated by pellicle), VNP (disks placed in a vertical position and uncoated by 
      pellicle), HCP (disks placed in a horizontal position and coated by pellicle), 
      and VCP (disks placed in a vertical position and coated by pellicle). Disks were 
      placed in a 24-well plate and a suspension of 107 cells/mL of Candida albicans 
      was added to each well for biofilm development. The plates were aerobically 
      incubated at 35 degrees C. The biofilms were evaluated at 1.5 (adhesion time point), 24, 
      48, 72, and 96 hours. The number of viable cells was quantified in terms of the 
      colony-forming units per milliliter (CFU/mL). Metabolic activity was measured by 
      the XTT assay. The biofilm structure was analyzed by scanning electron 
      microscopy. The data were analyzed by three-way ANOVA followed by Tukey's test, 
      with significance set at 5%. The vertical groups showed less biofilm formation 
      and lower metabolic activity than the horizontal groups (p<0.05). Significant 
      differences in cell viability and metabolic activity were observed between the 
      adhesion and other time points (p<0.05), but these variables were not affected by 
      the presence of the pellicle (p>0.05). It can be concluded that the substratum 
      position influenced biofilm development.
FAU - Cavalcanti, Indira Moraes Gomes
AU  - Cavalcanti IM
AD  - Univ of Campinas-UNICAMP, Piracicaba Dental School, Department of Prosthodontics 
      and Periodontology, Piracicaba, SP, Brazil.
FAU - Silva, Wander Jose da
AU  - Silva WJ
FAU - Lucena, Silvia Carneiro de
AU  - Lucena SC
FAU - Pousa, Camila Cordeiro
AU  - Pousa CC
FAU - Del Bel Cury, Altair Antoninha
AU  - Del Bel Cury AA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Brazil
TA  - Braz Oral Res
JT  - Brazilian oral research
JID - 101307187
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Analysis of Variance
MH  - Biofilms/*growth & development
MH  - Candida albicans/*growth & development
MH  - Colony-Forming Units Assay
MH  - Dental Pellicle/*microbiology
MH  - Microscopy, Electron, Scanning
MH  - Polymethyl Methacrylate
MH  - Random Allocation
MH  - Saliva/*microbiology
MH  - Surface Properties
MH  - Time Factors
EDAT- 2013/06/20 06:00
MHDA- 2014/01/18 06:00
CRDT- 2013/06/20 06:00
PHST- 2012/11/13 00:00 [received]
PHST- 2013/04/03 00:00 [accepted]
PHST- 2013/06/20 06:00 [entrez]
PHST- 2013/06/20 06:00 [pubmed]
PHST- 2014/01/18 06:00 [medline]
AID - S1806-83242013005000020 [pii]
AID - 10.1590/S1806-83242013005000020 [doi]
PST - ppublish
SO  - Braz Oral Res. 2013 Jul-Aug;27(4):369-75. doi: 10.1590/S1806-83242013005000020.