PMID- 23784732
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20181203
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 154
IP  - 2
DP  - 2013 Aug
TI  - Influence of selenium on mast cell mediator release.
PG  - 299-303
LID - 10.1007/s12011-013-9712-x [doi]
AB  - Selenium supplementation still enhanced the immune response even in individuals 
      who, according to current standards, would be considered as not being overtly 
      selenium deficient. Mast cells are granulated cells that play a pivotal role in 
      allergic reactions. In this study, we investigated the modulatory effect of 
      sodium selenite on mediator release and degranulation of murine mast cell line 
      (MC/9). Cells were pre-treated with selenium selenite (1, 2, 3 mug/ml) for 24 h 
      and controls left untreated. Then, cells were sensitized overnight with 
      anti-dinitrophenyl (DNP) IgE and challenged with DNP/HSA for degranulation 
      induction. The histamine and prostaglandin D2 (PGD2) were measured by ELISA, and 
      beta-hexosaminidase was measured by spectrophotometery method. Selenium-treated 
      cells revealed significant decrease in concentration of PGD2 (P = 0.019) and 
      beta-hexosaminidase (P = 0.009). In addition, a slight reduction of histamine 
      release by the selenium-treated cells was observed, based on our intracellular 
      and extracellular assessments. The most inhibitory effect of selenium 
      supplementation on mediator release of MC/9 cells was obtained in the presence of 
      3 mug/ml of sodium selenite. The results of the present study demonstrate 
      beneficial effects of supplemental selenium in attenuating clinical 
      manifestations of allergy and asthma.
FAU - Safaralizadeh, Reza
AU  - Safaralizadeh R
AD  - Department of Animal Biology, Faculty of Natural Science, University of Tabriz, 
      29 Bahman Blvd, Tabriz, Iran.
FAU - Nourizadeh, Maryam
AU  - Nourizadeh M
FAU - Zare, Ahad
AU  - Zare A
FAU - Kardar, Gholam Ali
AU  - Kardar GA
FAU - Pourpak, Zahra
AU  - Pourpak Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130620
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Trace Elements)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - H6241UJ22B (Selenium)
RN  - HIW548RQ3W (Sodium Selenite)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Animals
MH  - Asthma/drug therapy/metabolism/pathology
MH  - Cell Degranulation/*drug effects/immunology
MH  - Cell Line
MH  - Mast Cells/*metabolism/pathology
MH  - Mice
MH  - Prostaglandin D2/*metabolism
MH  - Selenium/pharmacology
MH  - Sodium Selenite/*pharmacology
MH  - Trace Elements/*pharmacology
MH  - beta-N-Acetylhexosaminidases/*metabolism
EDAT- 2013/06/21 06:00
MHDA- 2014/02/05 06:00
CRDT- 2013/06/21 06:00
PHST- 2013/02/15 00:00 [received]
PHST- 2013/05/20 00:00 [accepted]
PHST- 2013/06/21 06:00 [entrez]
PHST- 2013/06/21 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
AID - 10.1007/s12011-013-9712-x [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2013 Aug;154(2):299-303. doi: 10.1007/s12011-013-9712-x. 
      Epub 2013 Jun 20.