PMID- 23784965
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20181202
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 10
IP  - 7
DP  - 2016 Jul
TI  - Temporal profiling of the growth and multi-lineage potentiality of adipose 
      tissue-derived mesenchymal stem cells cell-sheets.
PG  - 564-79
LID - 10.1002/term.1776 [doi]
AB  - Cell-sheet tissue engineering retains the benefits of an intact extracellular 
      matrix (ECM) and can be used to produce scaffold-free constructs. Adipose 
      tissue-derived stem cells (ASCs) are multipotent and more easily obtainable than 
      the commonly used bone marrow-derived stem cells (BMSCs). Although BMSC cell 
      sheets have been previously reported to display multipotentiality, a detailed 
      study of the development and multilineage potential of ASC cell sheets (ASC-CSs) 
      is non-existent in the literature. The aims of this study were to temporally 
      profile: (a) the effect of hyperconfluent culture duration on ASC-CSs 
      development; and (b) the multipotentiality of ASC-CSs by differentiation into the 
      osteogenic, adipogenic and chondrogenic lineages. Rabbit ASCs were first isolated 
      and cultured until confluence (day 0). The confluent cells were then cultured in 
      ascorbic acid-supplemented medium for 3 weeks to study cell metabolic activity, 
      cell sheet thickness and early differentiation gene expressions at weekly time 
      points. ASC-CSs and ASCs were then differentiated into the three lineages, using 
      established protocols, and assessed by RT-PCR and histology at multiple time 
      points. ASC-CSs remained healthy up to 3 weeks of hyperconfluent culture. One 
      week-old cell sheets displayed upregulation of early differentiation gene markers 
      (Runx2 and Sox9); however, subsequent differentiation results indicated that they 
      did not necessarily translate to an improved phenotype. ASCs within the preformed 
      cell sheet groups did not differentiate as efficiently as the non-hyperconfluent 
      ASCs, which were directly differentiated. Although ASCs within the cell sheets 
      retained their differentiation capacity and remained viable under prolonged 
      hyperconfluent conditions, future applications of ASC-CSs in tissue engineering 
      should be considered with care. Copyright (c) 2016 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Neo, Puay Yong
AU  - Neo PY
AD  - Department of Bioengineering, Faculty of Engineering, National University of 
      Singapore.
FAU - See, Eugene Yong-Shun
AU  - See EY
AD  - Department of Bioengineering, Faculty of Engineering, National University of 
      Singapore.
FAU - Toh, Siew Lok
AU  - Toh SL
AD  - Department of Bioengineering, Faculty of Engineering, National University of 
      Singapore.
AD  - Department of Mechanical Engineering, Faculty of Engineering, National University 
      of Singapore.
FAU - Goh, James Cho-Hong
AU  - Goh JC
AD  - Department of Bioengineering, Faculty of Engineering, National University of 
      Singapore.
AD  - Department of Orthopaedic Surgery, Yong Lin Loo School of Medicine, National 
      University of Singapore.
LA  - eng
PT  - Journal Article
DEP - 20130620
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (SOX9 Transcription Factor)
SB  - IM
MH  - Adipose Tissue/cytology/*metabolism
MH  - Animals
MH  - Antigens, Differentiation/biosynthesis
MH  - *Cell Differentiation
MH  - Core Binding Factor Alpha 1 Subunit/biosynthesis
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Rabbits
MH  - SOX9 Transcription Factor/biosynthesis
MH  - Up-Regulation
OTO - NOTNLM
OT  - adipose-tissue derived stem cells
OT  - cell sheet
OT  - hyperconfluent culture
OT  - multi-lineage differentiation
OT  - temporal profiling
OT  - tissue engineering
EDAT- 2013/06/21 06:00
MHDA- 2017/11/29 06:00
CRDT- 2013/06/21 06:00
PHST- 2012/07/16 00:00 [received]
PHST- 2013/04/11 00:00 [revised]
PHST- 2013/04/16 00:00 [accepted]
PHST- 2013/06/21 06:00 [entrez]
PHST- 2013/06/21 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - 10.1002/term.1776 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2016 Jul;10(7):564-79. doi: 10.1002/term.1776. Epub 2013 
      Jun 20.