PMID- 23785195
OWN - NLM
STAT- MEDLINE
DCOM- 20131125
LR  - 20240104
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 17
DP  - 2013 Sep
TI  - An intact sialoadhesin (Sn/SIGLEC1/CD169) is not required for 
      attachment/internalization of the porcine reproductive and respiratory syndrome 
      virus.
PG  - 9538-46
LID - 10.1128/JVI.00177-13 [doi]
AB  - Surface expression of SIGLEC1, also known as sialoadhesin or CD169, is considered 
      a primary determinant of the permissiveness of porcine alveolar macrophages for 
      infection by porcine reproductive and respiratory syndrome virus (PRRSV). In 
      vitro, the attachment and internalization of PRRSV are dependent on the 
      interaction between sialic acid on the virion surface and the sialic acid binding 
      domain of the SIGLEC1 gene. To test the role of SIGLEC1 in PRRSV infection, a 
      SIGLEC1 gene knockout pig was created by removing part of exon 1 and all of exons 
      2 and 3 of the SIGLEC1 gene. The resulting knockout ablated SIGLEC1 expression on 
      the surface of alveolar macrophages but had no effect on the expression of CD163, 
      a coreceptor for PRRSV. After infection, PRRSV viremia in SIGLEC1(-/-) pigs 
      followed the same course as in SIGLEC1(-/+) and SIGLEC1(+/+) littermates. The 
      absence of SIGLEC1 had no measurable effect on other aspects of PRRSV infection, 
      including clinical disease course and histopathology. The results demonstrate 
      that the expression of the SIGLEC1 gene is not required for infection of pigs 
      with PRRSV and that the absence of SIGLEC1 does not contribute to the 
      pathogenesis of acute disease.
FAU - Prather, Randall S
AU  - Prather RS
AD  - Division of Animal Science, College of Food Agriculture and Natural Resources, 
      University of Missouri, Columbia, Missouri, USA. pratherr@missouri.edu
FAU - Rowland, Raymond R R
AU  - Rowland RR
FAU - Ewen, Catherine
AU  - Ewen C
FAU - Trible, Benjamin
AU  - Trible B
FAU - Kerrigan, Maureen
AU  - Kerrigan M
FAU - Bawa, Bhupinder
AU  - Bawa B
FAU - Teson, Jennifer M
AU  - Teson JM
FAU - Mao, Jiude
AU  - Mao J
FAU - Lee, Kiho
AU  - Lee K
FAU - Samuel, Melissa S
AU  - Samuel MS
FAU - Whitworth, Kristin M
AU  - Whitworth KM
FAU - Murphy, Clifton N
AU  - Murphy CN
FAU - Egen, Tina
AU  - Egen T
FAU - Green, Jonathan A
AU  - Green JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130619
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Antigens, CD/physiology
MH  - Antigens, Differentiation, Myelomonocytic/physiology
MH  - Gene Knockout Techniques
MH  - Host-Pathogen Interactions/immunology/physiology
MH  - Macrophages, Alveolar/immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/pathogenicity/*physiology
MH  - Receptors, Cell Surface/physiology
MH  - Sialic Acid Binding Ig-like Lectin 1/deficiency/genetics/*physiology
MH  - Sus scrofa
MH  - Swine
MH  - Virus Attachment
MH  - Virus Internalization
PMC - PMC3754101
EDAT- 2013/06/21 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/03/01
CRDT- 2013/06/21 06:00
PHST- 2013/06/21 06:00 [entrez]
PHST- 2013/06/21 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - JVI.00177-13 [pii]
AID - 00177-13 [pii]
AID - 10.1128/JVI.00177-13 [doi]
PST - ppublish
SO  - J Virol. 2013 Sep;87(17):9538-46. doi: 10.1128/JVI.00177-13. Epub 2013 Jun 19.