PMID- 23785990
OWN - NLM
STAT- MEDLINE
DCOM- 20141219
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 3
DP  - 2013 Mar
TI  - [Determination of nine beta-agonist residues in pig tissues by liquid 
      chromatography-tandem mass spectrometry combining with library search].
PG  - 200-5
AB  - A new method has been developed using a hybrid triple-quadrupole linear ion trap 
      (QTrap) mass spectrometer for the fast detection and identification of nine 
      beta-agonists, clenbuterol, salbutamol, ractopamine, ritodrine, terbutaline, 
      isoxsuprine, tulobuterol, cimaterol and bambuterol, in one single liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The homogenized 
      tissue samples were purified with liquid-liquid extraction after enzymatic 
      hydrolysis by P-glucuronidase/aryl sulfatase. After gradient elution separation 
      on C(18) LC column using acetonitrile and formic acid aqueous solution as the 
      mobile phases, a multiple reaction monitoring (MRM) scan as survey scan and an 
      enhanced product ion (EPI) scan as dependent scan were performed in an 
      information dependent acquisition (IDA) experiment. Finally, the identification 
      of the drugs was carried out by library search with a newly developed MS/MS 
      library based on EPI spectra at three different collision energies in positive 
      mode. The analytical method in the present study was well validated and good 
      results were obtained with respect to precision, repeatability and spiked 
      recovery. The limits of detection of residues were 0.1 -0.2 micro g/kg for 
      beta-agonists, and with a linear range from 0.1 to 50.0 micro g/L. Three 
      concentration levels of 0. 5, 1. 0 and 5. 0 pg/kg were spiked in pig tissues, and 
      the overall recoveries were between 72.0% and 95.1% with the relative standard 
      deviations (RSDs) between 3. 1% and 12.1%. The real sample test showed that this 
      method could be used for sensitive and accurate determination of beta-agonist 
      residues in pig tissue
FAU - Cai, Qinren
AU  - Cai Q
AD  - Technical Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 
      519015, China. mr.cai@vip.163.com
FAU - Wu, Jieshan
AU  - Wu J
FAU - Qian, Zhenjie
AU  - Qian Z
FAU - Peng, Yufen
AU  - Peng Y
FAU - Cai, Jie
AU  - Cai J
FAU - Du, Zhifeng
AU  - Du Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Phenethylamines)
RN  - 57370OZ3P1 (ractopamine)
RN  - QF8SVZ843E (Albuterol)
RN  - XTZ6AXU7KN (Clenbuterol)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis
MH  - Albuterol/analysis
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Clenbuterol/analysis
MH  - Drug Residues/*analysis
MH  - Food Analysis/methods
MH  - Food Contamination/*analysis
MH  - Meat/analysis
MH  - Phenethylamines/analysis
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/06/22 06:00
MHDA- 2014/12/20 06:00
CRDT- 2013/06/22 06:00
PHST- 2013/06/22 06:00 [entrez]
PHST- 2013/06/22 06:00 [pubmed]
PHST- 2014/12/20 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.11004 [doi]
PST - ppublish
SO  - Se Pu. 2013 Mar;31(3):200-5. doi: 10.3724/sp.j.1123.2012.11004.