PMID- 237890
OWN - NLM
STAT- MEDLINE
DCOM- 19751010
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 250
IP  - 1
DP  - 1975 Jan 10
TI  - Kinetic study by pulse radiolysis of the lactate dehydrogenase-catalyzed chain 
      oxidation of nicotinamide adenine dinucleotide by HO2 and O2-RADICALS.
PG  - 318-21
AB  - The lactate dehydrogenase-catalyzed chain oxidation of NADH (LDH-NADH) by the 
      superoxide radicals, HO2 and O2, has been studied with pulse radiolysis in the pH 
      range between 4.5 and 9.0. The rate constants for the oxidation of the LDH-NADH 
      by HO2 and O2 determined at 23 degrees are 1.2 times 10-6 M(-1) s(-1) and 3.6 
      times 10-4 M(-1) s(-1), respectively. The latter represents an activation of over 
      1000-fold by the enzyme. A chain reaction mechanism consistent with the results 
      from these kinetic studies has been proposed.
FAU - Bielski, B H
AU  - Bielski BH
FAU - Chan, P C
AU  - Chan PC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Free Radicals)
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Enzyme Activation
MH  - Free Radicals
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - L-Lactate Dehydrogenase/*metabolism
MH  - Mathematics
MH  - Myocardium/enzymology
MH  - *NAD
MH  - Oxidation-Reduction
MH  - *Oxygen
MH  - Protein Binding
MH  - Spectrophotometry, Ultraviolet
MH  - Swine
MH  - Temperature
MH  - Time Factors
EDAT- 1975/01/10 00:00
MHDA- 1975/01/10 00:01
CRDT- 1975/01/10 00:00
PHST- 1975/01/10 00:00 [pubmed]
PHST- 1975/01/10 00:01 [medline]
PHST- 1975/01/10 00:00 [entrez]
AID - S0021-9258(19)42017-6 [pii]
PST - ppublish
SO  - J Biol Chem. 1975 Jan 10;250(1):318-21.