PMID- 23789227 OWN - NLM STAT- MEDLINE DCOM- 20130716 LR - 20220321 IS - 0255-2922 (Print) IS - 0255-2922 (Linking) VI - 33 IP - 2 DP - 2013 Apr TI - Effect of electroacupuncture on brain-derived neurotrophic factor mRNA expression in mouse hippocampus following cerebral ischemia-reperfusion injury. PG - 253-7 AB - OBJECTIVE: This work aims to observe the effects of electroacupuncture on brain-derived neurotrophic factor (BDNF) mRNA expression in mouse hippocampus following cerebral ischemia-reperfusion injury. METHODS: The models of mouse cerebral ischemia-reperfusion injury were established. A total of 96 healthy mice were randomly assigned into 4 groups, namely, the sham surgery, model, model + electroacupuncture, and mode + hydergine groups. Mice in the model + electroacupuncture group were treated through electroacupuncture at the Shenshu (BL 23), Geshu (BL 17), and Baihui (GV 20) acupoints. Mice in the model+hydergine group were intragastrically administered with hydergine (0.77 mg/kg(-1) x day(-1)). The levels of BDNF mRNA expressions in the hippocampus were ana lyzed through a semi-quantitative reverse transcription-polymerase chain reaction assay on days 1 and 7 after the surgeries. RESULTS: BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group (both P < 0.01). On days 1 and 7 of the electroacupuncture treatment, BDNF mRNA expression in the mouse hippocampus of the model + electroacupuncture group was significantly elevated compared with the model group (both P < 0.01) or the model + hydergine group (both P < 0.01). On days 1 and 7 of the hydergine treatment, BDNF mRNA expression in the mouse hippocampus of the model + hydergine group tended to increase compared with the model group; however, statistical significance was not achieved (both P > 0.05). CONCLUSION: Electroacupuncture treatment enhances endogenous BDNF expression, which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells. FAU - Zhao, Jianxin AU - Zhao J AD - The Third Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing 100029, China. FAU - Xu, Huazhou AU - Xu H FAU - Tian, Yuanxiang AU - Tian Y FAU - Hu, Manxiang AU - Hu M FAU - Xiao, Hongling AU - Xiao H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - J Tradit Chin Med JT - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JID - 8211546 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) SB - IM MH - Acupuncture Points MH - Animals MH - Brain Ischemia/genetics/metabolism/*surgery MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - *Electroacupuncture MH - Hippocampus/*metabolism MH - Humans MH - Male MH - Mice MH - RNA, Messenger/genetics/metabolism MH - Reperfusion Injury/*genetics/metabolism/*therapy EDAT- 2013/06/26 06:00 MHDA- 2013/07/17 06:00 CRDT- 2013/06/25 06:00 PHST- 2013/06/25 06:00 [entrez] PHST- 2013/06/26 06:00 [pubmed] PHST- 2013/07/17 06:00 [medline] AID - 10.1016/s0254-6272(13)60135-1 [doi] PST - ppublish SO - J Tradit Chin Med. 2013 Apr;33(2):253-7. doi: 10.1016/s0254-6272(13)60135-1.