PMID- 23790156
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20181202
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 70
IP  - 4
DP  - 2013 Oct
TI  - TLR7 expression is decreased during tumour progression in transgenic 
      adenocarcinoma of mouse prostate mice and its activation inhibits growth of 
      prostate cancer cells.
PG  - 317-26
LID - 10.1111/aji.12146 [doi]
AB  - PROBLEM: Although various Toll-like receptors (TLRs) have been associated with 
      immune response and tumorigenesis in the prostate cells, little is known about 
      the role of TLR7. Accordingly, we examined the expression of TLR7 during tumour 
      progression of TRMAP (transgenic mouse model for prostate cancer) mice and its 
      role on cell growth. METHOD OF STUDY: Toll-like receptor7 expression was examined 
      by RT-polymerase chain reaction (PCR), Western blot, and immunohistochemistry. 
      Cell growth was examined by MTT assay. Colony formation was investigated by 
      crystal violet staining. RESULTS: Strong expression of TLR7 was detected in the 
      normal prostate epithelia of Wild-type (WT) mice, but not in TLR7-deficient mice. 
      In contrast, TLR7 expression was weak in transgenic adenocarcinoma of mouse 
      prostate (TRAMP)-C2 cells, as compared with murine bone marrow-derived 
      macrophages (BMDMs). Moreover, TLR7 mRNA was markedly expressed in RWPE-1 cells 
      (non-cancerous prostate epithelial cells), but not in PC3 and DU145 (prostate 
      cancer cells). Immunohistochemically, TLR7 expression gradually decreased in 
      TRAMP mice depending on the pathologic grade of the prostate cells. TLR7 agonists 
      increased both the gene and protein expression of TLR7 and promoted production of 
      proinflammatory cytokines/chemokines and IFN-beta gene expression in prostate cancer 
      cell lines. Moreover, loxoribine inhibited the growth and colony formation of 
      TRAMP-C2 cells dependent of TLR7. CONCLUSION: These findings suggest that TLR7 
      may participate in tumour suppression in the prostate cells.
CI  - (c) 2013 John Wiley & Sons Ltd.
FAU - Han, Ju-Hee
AU  - Han JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Seoul University, 
      Seoul, Korea.
FAU - Park, Shin-Young
AU  - Park SY
FAU - Kim, Jin-Bum
AU  - Kim JB
FAU - Cho, Sung-Dae
AU  - Cho SD
FAU - Kim, Bumseok
AU  - Kim B
FAU - Kim, Bo-Yeon
AU  - Kim BY
FAU - Kang, Min-Jung
AU  - Kang MJ
FAU - Kim, Dong-Jae
AU  - Kim DJ
FAU - Park, Jae-Hak
AU  - Park JH
FAU - Park, Jong-Hwan
AU  - Park JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130624
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Aminoquinolines)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Toll-Like Receptor 7)
RN  - 12133JR80S (Guanosine)
RN  - 9CAS0V66OI (loxoribine)
RN  - P1QW714R7M (Imiquimod)
SB  - IM
MH  - Adenocarcinoma/drug therapy/genetics/*immunology
MH  - Aminoquinolines/pharmacology
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Carcinogenesis/drug effects/genetics
MH  - Cell Growth Processes/drug effects/genetics
MH  - Cell Line, Tumor
MH  - Cytokines/genetics/metabolism
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Epithelial Cells/*immunology
MH  - Gene Expression Regulation, Neoplastic/drug effects/genetics
MH  - Guanosine/analogs & derivatives/pharmacology
MH  - Humans
MH  - Imiquimod
MH  - Inflammation Mediators/metabolism
MH  - Macrophages/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Prostate/*pathology
MH  - Prostatic Neoplasms/drug therapy/genetics/*immunology
MH  - Toll-Like Receptor 7/agonists/genetics/*metabolism
MH  - Transgenes/genetics
MH  - Tumor Stem Cell Assay
OTO - NOTNLM
OT  - Colony formation
OT  - TLR7
OT  - growth inhibition
OT  - prostate cancer
OT  - transgenic adenocarcinoma of mouse prostate mice
EDAT- 2013/06/25 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/06/25 06:00
PHST- 2013/02/08 00:00 [received]
PHST- 2013/06/04 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - 10.1111/aji.12146 [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2013 Oct;70(4):317-26. doi: 10.1111/aji.12146. Epub 2013 Jun 
      24.