PMID- 23790854
OWN - NLM
STAT- MEDLINE
DCOM- 20140103
LR  - 20191210
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 141
IP  - 2
DP  - 2013 Nov 15
TI  - An HPLC-DAD method for the simultaneous determination of nine beta-lactam 
      antibiotics in ewe milk.
PG  - 829-34
LID - S0308-8146(13)00387-7 [pii]
LID - 10.1016/j.foodchem.2013.02.131 [doi]
AB  - The presence of beta-lactam residues in foodstuffs constitutes a potential risk to 
      the human health and undesirable effects on consumers, and nowadays these 
      antibiotic residues are also recognised as an emerging environmental problem. In 
      addition, these are of great concern to prestigious Manchego cheese processors 
      (Central Spain denomination of origin) because they reduce the curdling of milk 
      and cause improper cheese ripening, which consequently lead to an important loss 
      of monetary income. This work describes the development of a sensitive and 
      reliable method using liquid chromatography with UV-diode array detection 
      (LC-DAD) for simultaneous determination of the beta-lactam antibiotics, ampicillin 
      (AMP), benzylpenicillin (PEG), cephalexin (CFX), cefazolin (CFL), cefoperazone 
      (CFP), cloxacillin (CLO), dicloxacillin (DCL), oxacillin (OXA) and 
      phenoxymethylpenicillin (PEV), in Manchega ewe milk. The column, mobile phase, 
      temperature and flow rate were optimised to provide the best resolution of these 
      analytes. The extraction method of the antibiotic residues involves the 
      deproteinisation of the milk sample using acetonitrile and centrifugation 
      followed by a solid-phase extraction (SPE) clean-up. The recoveries for the 
      studied beta-lactams ranged from 79% to 96% with relative standard deviations 
      between 0.5% and 4.9%. The limits of quantification (LOQs) for all these 
      compounds were in the range of 3.4-8.6mugkg(-1), which are lower than the maximum 
      residue limits (MRLs) established by the European Union for the studied beta-lactams 
      in milk, making the method suitable for performing routine analyses. The proposed 
      multi-residue LC-UV-diode array detection (LC-DAD) method is a powerful and 
      popular alternative for the determination and confirmation of antibiotic residues 
      in small milk industries and is the first one capable of determining nine 
      beta-lactam antibiotics in samples of Manchega ewe milk.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Camara, M
AU  - Camara M
AD  - Regional Centre of Animal Selection and Reproduction (CERSYRA), E-13300 
      Valdepenas, Spain.
FAU - Gallego-Pico, A
AU  - Gallego-Pico A
FAU - Garcinuno, R M
AU  - Garcinuno RM
FAU - Fernandez-Hernando, P
AU  - Fernandez-Hernando P
FAU - Durand-Alegria, J S
AU  - Durand-Alegria JS
FAU - Sanchez, P J
AU  - Sanchez PJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130328
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (beta-Lactams)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Food Contamination/*analysis
MH  - Milk/*chemistry
MH  - beta-Lactams/*analysis
EDAT- 2013/06/26 06:00
MHDA- 2014/01/05 06:00
CRDT- 2013/06/25 06:00
PHST- 2011/03/04 00:00 [received]
PHST- 2012/10/24 00:00 [revised]
PHST- 2013/02/22 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/26 06:00 [pubmed]
PHST- 2014/01/05 06:00 [medline]
AID - S0308-8146(13)00387-7 [pii]
AID - 10.1016/j.foodchem.2013.02.131 [doi]
PST - ppublish
SO  - Food Chem. 2013 Nov 15;141(2):829-34. doi: 10.1016/j.foodchem.2013.02.131. Epub 
      2013 Mar 28.