PMID- 23791734
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20181202
IS  - 1873-3476 (Electronic)
IS  - 0378-5173 (Linking)
VI  - 454
IP  - 2
DP  - 2013 Oct 1
TI  - Protein entrapment in PEGylated lipid nanoparticles.
PG  - 625-32
LID - S0378-5173(13)00495-X [pii]
LID - 10.1016/j.ijpharm.2013.06.006 [doi]
AB  - Defining appropriate delivery strategies of therapeutic proteins, based on lipid 
      nanoparticulate carriers, requires knowledge of the nanoscale organization that 
      determines the loading and release properties of the nanostructured particles. 
      Nanoencapsulation of three cationic proteins (human brain-derived neurotrophic 
      factor (BDNF), alpha-chymotrypsinogen A, and histone H3) was investigated using 
      anionic nanoparticle (NP) carriers. PEGylated lipid NPs were prepared from 
      self-assembled liquid crystalline phases involving monoolein and eicosapentaenoic 
      acid. Inclusion of the antioxidant alpha-tocopherol favoured the preparation of 
      stealth hexosome carriers. The purpose of the present work is to reveal the 
      structural features of the protein-loaded lipid nanocarriers by means of high 
      resolution small-angle X-ray scattering (SAXS) and cryogenic transmission 
      electron microscopy (cryo-TEM). The obtained results indicate that protein 
      entrapment is concentration-dependent and may significantly modify the inner 
      liquid crystalline structure of the lipid nanocarriers through changes in the 
      interfacial curvature and hydration.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Angelova, Angelina
AU  - Angelova A
AD  - CNRS UMR8612, Institut Galien Paris-Sud, 5 rue J.B. Clement, F-92296 
      Chatenay-Malabry Cedex, France. Angelina.Angelova@u-psud.fr
FAU - Angelov, Borislav
AU  - Angelov B
FAU - Drechsler, Markus
AU  - Drechsler M
FAU - Garamus, Vasil M
AU  - Garamus VM
FAU - Lesieur, Sylviane
AU  - Lesieur S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130617
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Histones)
RN  - 0 (Lipids)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9035-75-0 (Chymotrypsinogen)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*chemistry
MH  - Chymotrypsinogen/*chemistry
MH  - Histones/*chemistry
MH  - Lipids/chemistry
MH  - Microscopy, Electron, Transmission
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Polyethylene Glycols/chemistry
MH  - Scattering, Small Angle
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - BDNF
OT  - Hexosomes
OT  - Neurotrophin
OT  - PEGylated liquid crystalline nanocarriers
OT  - Protein nanoencapsulation
EDAT- 2013/06/25 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/25 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/06/03 00:00 [revised]
PHST- 2013/06/06 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - S0378-5173(13)00495-X [pii]
AID - 10.1016/j.ijpharm.2013.06.006 [doi]
PST - ppublish
SO  - Int J Pharm. 2013 Oct 1;454(2):625-32. doi: 10.1016/j.ijpharm.2013.06.006. Epub 
      2013 Jun 17.