PMID- 23791972
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20181202
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 17
IP  - 2
DP  - 2013 Oct
TI  - Mesenchymal stem cells transplantation ameliorates glomerular injury in 
      streptozotocin-induced diabetic nephropathy in rats via inhibiting macrophage 
      infiltration.
PG  - 275-82
LID - S1567-5769(13)00232-4 [pii]
LID - 10.1016/j.intimp.2013.05.031 [doi]
AB  - Mesenchymal stem cells (MSCs) treatment has been shown to be effective in 
      diabetic nephropathy (DN). However, the mechanisms involved in the renoprotective 
      effects of MSCs have not been clearly demonstrated. Especially, there was no 
      study on the relationship of MSCs and macrophages in diabetic kidney. To explore 
      the effect of MSCs on macrophages in DN, streptozotocin-induced diabetes animals 
      received no treatment or treatment with MSCs (2x10(6), via tail vein) for two 
      continuous weeks. Eight weeks after treatment, physical, biochemical and 
      morphological parameters were measured. Immunohistochemistry for fibronectin 
      (FN), CollagenI, ED-1, monocyte chemoattractant protein-1 (MCP-1) was performed. 
      Expressions of pro-inflammatory cytokines and hepatocyte growth factor (HGF) at 
      gene level and protein level were determined by real-time reverse 
      transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, 
      respectively. Blood glucose, urinary albumin excretion, creatinine clearance were 
      significantly reduced after MSCs treatment. The glomerulosclerosis as revealed by 
      periodic acid Schiff stain and expression of FN and CollagenI was also 
      dramatically attenuated. Most importantly, the expression of MCP-1 and the number 
      of infiltrated macrophages in kidney were effectively suppressed by MSCs 
      treatment. The expression of HGF in MSCs group was up-regulated. Meanwhile, the 
      expressions of IL-1beta, IL-6 and TNFalpha were significantly down-regulated by MSCs 
      treatment. Our study suggest that MSCs treatment ameliorates DN via inhibition of 
      MCP-1 expression by secreting HGF, thus reducing macrophages infiltration, 
      down-regulating IL-1beta, IL-6, TNFalpha expression in renal tissue in diabetic rats.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Lv, Sha-Sha
AU  - Lv SS
AD  - Shandong University, Jinan, Shandong, China.
FAU - Liu, Gang
AU  - Liu G
FAU - Wang, Jian-Ping
AU  - Wang JP
FAU - Wang, Wei-Wei
AU  - Wang WW
FAU - Cheng, Jing
AU  - Cheng J
FAU - Sun, Ai-Li
AU  - Sun AL
FAU - Liu, Hai-Ying
AU  - Liu HY
FAU - Nie, Hui-Bin
AU  - Nie HB
FAU - Su, Mo-Ran
AU  - Su MR
FAU - Guan, Guang-Ju
AU  - Guan GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130619
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Chemokine CCL2)
RN  - 0 (Collagen Type I)
RN  - 0 (Cytokines)
RN  - 0 (Fibronectins)
RN  - 0 (Inflammation Mediators)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Collagen Type I/metabolism
MH  - Creatinine/urine
MH  - Cytokines/genetics/metabolism
MH  - Diabetes Mellitus, Experimental/immunology/*therapy
MH  - Diabetic Nephropathies/immunology/*therapy
MH  - Down-Regulation
MH  - Female
MH  - Fibronectins/metabolism
MH  - Hepatocyte Growth Factor/metabolism
MH  - Inflammation Mediators/metabolism
MH  - Kidney/*metabolism/pathology
MH  - Macrophages/*pathology
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - Diabetic rats
OT  - Inflammation
OT  - Macrophages
OT  - Nephropathy
EDAT- 2013/06/25 06:00
MHDA- 2014/04/23 06:00
CRDT- 2013/06/25 06:00
PHST- 2013/04/16 00:00 [received]
PHST- 2013/05/13 00:00 [revised]
PHST- 2013/05/27 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
AID - S1567-5769(13)00232-4 [pii]
AID - 10.1016/j.intimp.2013.05.031 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2013 Oct;17(2):275-82. doi: 10.1016/j.intimp.2013.05.031. 
      Epub 2013 Jun 19.