PMID- 23792237 OWN - NLM STAT- MEDLINE DCOM- 20140303 LR - 20130801 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 114 DP - 2013 Oct TI - Rapid discrimination of Herba Cistanches by multi-step infrared macro-fingerprinting combined with soft independent modeling of class analogy (SIMCA). PG - 421-31 LID - S1386-1425(13)00513-1 [pii] LID - 10.1016/j.saa.2013.05.024 [doi] AB - Herba Cistanche, an important Chinese herbal medicine, has common four species, Cistanche deserticola (CD), Cistanche tubulosa (CT), Cistanche salsa (CS) and Cistanche sinensis (CSN) which have been frequent mixed used. To clarify the sources of Herba Cistanches and ensure the clinical efficacy and safety, a method combing IR macro-fingerprinting with statistical pattern recognition was developed to analyze and discriminate the four species of Herba Cistanche. By comparing FT-IR, second derivative spectral fingerprints via group-peak matching, the similarity to CD and total saccharides (TS) followed an increasing sequence, CTCSN>CS>CD. Characteristic fingerprints of their 2D-IR correlation spectra in 1750-1000 cm(-1) have confirmed the above findings in a more intuitive way. In terms of sources for phenylethanoid glycosides (PhGs), CT can be an ideal alternative species. However, in terms of using them as a whole, more pharmacological study should be conducted due to the different ratios of their chemical constituents, which is also applicable to CSN and CS. Moreover, the four species (179 samples) has been objectively classified by SIMCA based on IR macro-fingerprints. CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Xu, Changhua AU - Xu C AD - Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Analysis Center, Tsinghua University, Beijing 100084, China. xuchanghua@gmail.com FAU - Jia, Xiaoguang AU - Jia X FAU - Xu, Rong AU - Xu R FAU - Wang, Yang AU - Wang Y FAU - Zhou, Qun AU - Zhou Q FAU - Sun, Suqin AU - Sun S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130531 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 RN - 0 (Drugs, Chinese Herbal) RN - 0 (Glycosides) SB - IM MH - Cistanche/*chemistry MH - Drugs, Chinese Herbal/*chemistry MH - Glycosides/analysis MH - Quality Control MH - Spectroscopy, Fourier Transform Infrared/*methods OTO - NOTNLM OT - Cistanche deserticola OT - Discrimination OT - Herba Cistanche OT - IR macro-fingerprint OT - SIMCA EDAT- 2013/06/25 06:00 MHDA- 2014/03/04 06:00 CRDT- 2013/06/25 06:00 PHST- 2012/11/07 00:00 [received] PHST- 2013/05/07 00:00 [revised] PHST- 2013/05/09 00:00 [accepted] PHST- 2013/06/25 06:00 [entrez] PHST- 2013/06/25 06:00 [pubmed] PHST- 2014/03/04 06:00 [medline] AID - S1386-1425(13)00513-1 [pii] AID - 10.1016/j.saa.2013.05.024 [doi] PST - ppublish SO - Spectrochim Acta A Mol Biomol Spectrosc. 2013 Oct;114:421-31. doi: 10.1016/j.saa.2013.05.024. Epub 2013 May 31.