PMID- 23793828
OWN - NLM
STAT- MEDLINE
DCOM- 20140225
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 40
IP  - 8
DP  - 2013 Aug
TI  - Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and 
      adiponectin expression.
PG  - 5027-34
LID - 10.1007/s11033-013-2603-6 [doi]
AB  - MicroRNAs are endogenous small RNAs with a high degree of conservation, 
      participating in a variety of vital activities. In present study, to explore the 
      effect of microRNAs on 3T3-L1 adipocyte differentiation and adiponectin 
      expression, the adipo-related microRNAs were screened and identified by micorRNA 
      microarray. The highly expression plasmid of microRNA-21 with obvious expression 
      up-regulation (miR-21) and its anti-sense (miR-21 inhibitor) were constructed and 
      transfected into 3T3-L1 preadipocytes. The effect of miR-21 on 3T3-L1 adipocyte 
      differentiation was observed, and the protein and mRNA expression level of 
      adiponectin and AP-1 were analyzed. Results showed that, the expression profiles 
      of microRNAs significantly changed during 3T3-L1 adipocyte differentiation. The 
      expression of miR-21 was obviously up-regulated. miR-21 could significantly 
      promote adipocyte differentiation, increase adiponectin mRNA and protein 
      expression, while decrease AP-1 protein level. Meanwhil, miR-21 inhibitor blocked 
      the effects of miR-21 mentioned above. The overexpression of AP-1 could 
      absolutely reverse the stimulatory effect of miR-21 on adiponectin. miR-21 plays 
      an important role in regulating adipocyte differentiation and adiponectin 
      expression by inhibiting AP-1 expression.
FAU - Kang, Min
AU  - Kang M
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, Nanning, 530021, China.
FAU - Yan, Li-Mei
AU  - Yan LM
FAU - Zhang, Wen-Yu
AU  - Zhang WY
FAU - Li, Yu-Mei
AU  - Li YM
FAU - Tang, An-Zhou
AU  - Tang AZ
FAU - Ou, He-Sheng
AU  - Ou HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130621
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Adiponectin)
RN  - 0 (DNA Primers)
RN  - 0 (MIRN21 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Transcription Factor AP-1)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/metabolism/*physiology
MH  - Adiponectin/*metabolism
MH  - Animals
MH  - Cell Differentiation/genetics/*physiology
MH  - DNA Primers/genetics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*genetics
MH  - Mice
MH  - MicroRNAs/*metabolism
MH  - Microarray Analysis
MH  - Transcription Factor AP-1/*metabolism
MH  - Transfection
EDAT- 2013/06/25 06:00
MHDA- 2014/02/26 06:00
CRDT- 2013/06/25 06:00
PHST- 2012/08/15 00:00 [received]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/02/26 06:00 [medline]
AID - 10.1007/s11033-013-2603-6 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2013 Aug;40(8):5027-34. doi: 10.1007/s11033-013-2603-6. Epub 2013 
      Jun 21.