PMID- 23793904
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 38
IP  - 9
DP  - 2013 Sep
TI  - Effect of HDAC inhibitors on neuroprotection and neurite outgrowth in primary rat 
      cortical neurons following ischemic insult.
PG  - 1921-34
LID - 10.1007/s11064-013-1098-9 [doi]
AB  - Histone deacetylase inhibitors (HDACi)-valproic acid (VPA) and trichostatin A 
      (TSA) promote neurogenesis, neurite outgrowth, synaptic plasticity and 
      neuroprotection. In this study, we investigated whether VPA and TSA promote 
      post-ischemic neuroprotection and neuronal restoration in rat primary cortical 
      neurons. On 6 days in vitro (DIV), cortical neurons were exposed to 
      oxygen-glucose deprivation for 90 min. Cells were returned to normoxic conditions 
      and cultured for 1, 3, or 7 days with or without VPA and TSA. Control cells were 
      cultured in normoxic conditions only. On 7, 9, and 13 DIV, cells were measured 
      neurite outgrowth using the Axiovision program and stained with Tunel staining 
      kit. Microtubule associated protein-2 immunostaining and tunel staining showed 
      significant recovery of neurite outgrowth and post-ischemic neuronal death by VPA 
      or TSA treatment. We also determined levels of acetylated histone H3, PSD95, GAP 
      43 and synaptophysin. Significant increases in all three synaptic markers and 
      acetylated histone H3 were observed relative to non-treated cells. Post-ischemic 
      HDACi treatment also significantly raised levels of brain derived neurotrophic 
      factor (BDNF) expression and secreted BDNF. Enhanced BDNF expression by HDACi 
      treatment might have been involved in the post-ischemic neuroprotection and 
      neuronal restorative effects. Our findings suggest that both VPA and TSA 
      treatment during reoxygenation after ischemia may help post-ischemic 
      neuroprotection and neuronal regeneration via increased BDNF expression and 
      activation.
FAU - Hasan, Mohammad Rakibul
AU  - Hasan MR
AD  - Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, 
      Konkuk University, Seoul, 143-701, Korea.
FAU - Kim, Ji-Hye
AU  - Kim JH
FAU - Kim, Youn Jung
AU  - Kim YJ
FAU - Kwon, Kyoung Ja
AU  - Kwon KJ
FAU - Shin, Chan Young
AU  - Shin CY
FAU - Kim, Hahn Young
AU  - Kim HY
FAU - Han, Seol-Heui
AU  - Han SH
FAU - Choi, Dong-Hee
AU  - Choi DH
FAU - Lee, Jongmin
AU  - Lee J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130622
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Neuroprotective Agents)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - DNA Primers
MH  - Female
MH  - Histone Deacetylase Inhibitors/*pharmacology
MH  - *Neurites
MH  - Neuroprotective Agents/*pharmacology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2013/06/25 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/25 06:00
PHST- 2013/02/26 00:00 [received]
PHST- 2013/06/13 00:00 [accepted]
PHST- 2013/05/28 00:00 [revised]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - 10.1007/s11064-013-1098-9 [doi]
PST - ppublish
SO  - Neurochem Res. 2013 Sep;38(9):1921-34. doi: 10.1007/s11064-013-1098-9. Epub 2013 
      Jun 22.