PMID- 23794058
OWN - NLM
STAT- MEDLINE
DCOM- 20140706
LR  - 20211021
IS  - 2095-0225 (Electronic)
IS  - 2095-0217 (Linking)
VI  - 7
IP  - 3
DP  - 2013 Sep
TI  - Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate 
      into sweat gland-like cells: a preclinical study.
PG  - 345-53
LID - 10.1007/s11684-013-0282-2 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various 
      advantageous properties, including self-renewal, extended proliferation 
      potential, multi-lineage differentiation potential and capacity for 
      differentiating into sweat gland-like cells in certain conditions. However, 
      little is known about the effect of clinical-grade culture conditions on these 
      properties and on the differentiative potential of hUC-MSCs. In this study, we 
      sought to investigate the properties of hUC-MSCs expanded with animal serum free 
      culture media (ASFCM) in order to determine their potential for differentiation 
      into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be 
      established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous 
      proliferation comparable to those of cells grown in classical culture conditions 
      containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was 
      comparable to those of cells grown under classical culture conditions, and 
      hUC-MSCs grown in both of the two culture conditions tested showed the typical 
      antigen profile of MSCs-positive for CD29, CD44, CD90, and CD105, and negative 
      for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the 
      cells were stable after long-term culture under both culture conditions. Like 
      normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited 
      expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA 
      and EDAR) that are characteristic of normal sweat gland cells. Taken together, 
      our findings indicate that the classical culture medium used to differentiate 
      hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical 
      purposes.
FAU - Yang, Siming
AU  - Yang S
AD  - Institute of Basic Medical Sciences, PLA General Hospital, the PLA Medical 
      College, Beijing, 100853, China.
FAU - Ma, Kui
AU  - Ma K
FAU - Feng, Changjiang
AU  - Feng C
FAU - Wu, Yan
AU  - Wu Y
FAU - Wang, Yao
AU  - Wang Y
FAU - Huang, Sha
AU  - Huang S
FAU - Fu, Xiaobing
AU  - Fu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130623
PL  - China
TA  - Front Med
JT  - Frontiers of medicine
JID - 101549428
SB  - IM
MH  - Cell Differentiation
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Sweat Glands/*cytology/metabolism
MH  - Umbilical Cord/*cytology
EDAT- 2013/06/25 06:00
MHDA- 2014/07/07 06:00
CRDT- 2013/06/25 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/06/25 06:00 [entrez]
PHST- 2013/06/25 06:00 [pubmed]
PHST- 2014/07/07 06:00 [medline]
AID - 10.1007/s11684-013-0282-2 [doi]
PST - ppublish
SO  - Front Med. 2013 Sep;7(3):345-53. doi: 10.1007/s11684-013-0282-2. Epub 2013 Jun 
      23.