PMID- 2379553
OWN - NLM
STAT- MEDLINE
DCOM- 19900907
LR  - 20190722
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 109
IP  - 2
DP  - 1990 Aug
TI  - Localization of brain-derived neurotrophic factor mRNA to neurons in the brain by 
      in situ hybridization.
PG  - 141-52
AB  - Brain-derived neurotrophic factor (BDNF) which supports survival of and fiber 
      outgrowth from sensory neurons and retinal ganglion cells has recently been 
      cloned. It is closely related to nerve growth factor (NGF), which we had 
      demonstrated earlier by in situ hybridization to be expressed in the brain by 
      cholinergic target neurons. Using oligonucleotide probes we now describe aspects 
      of the distribution of BDNF mRNA in the adult pig and rat brain. Similar to NGF, 
      BDNF expression in vivo in the central nervous system appeared to be mostly 
      confined to neurons. In the hippocampal formation, strongly labeled neurons were 
      found in and around the pyramidal layer, as well as in the granular layer and the 
      hilus of the dentate gyrus of both pig and rat. Other neurons in these areas were 
      more weakly labeled or unlabeled. High BDNF expression was also shown in 
      scattered neurons in cortex cerebri, in many neurons in claustrum and in certain 
      other areas. These results demonstrate that the similarity in structure between 
      NGF and BDNF is paralleled by a similarity in cellular expression, suggesting 
      that BDNF, like NGF, may act via a direct neuron-to-neuron interaction. It is 
      possible that some neurons, for example, in the hippocampal formation, express 
      both NGF and BDNF. Finally, the localization of BDNF expression in cortical areas 
      and in claustrum suggests that the target neurons for this factor extend beyond 
      the sensory ganglion and retinal ganglion cells.
FAU - Wetmore, C
AU  - Wetmore C
AD  - Department of Histology & Neurobiology, Karolinska Institute, Stockholm, Sweden.
FAU - Ernfors, P
AU  - Ernfors P
FAU - Persson, H
AU  - Persson H
FAU - Olson, L
AU  - Olson L
LA  - eng
GR  - AG-04418/AG/NIA NIH HHS/United States
GR  - NS-09199/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Basal Ganglia/metabolism
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cerebellum/metabolism
MH  - Cerebral Cortex/metabolism
MH  - Hippocampus/metabolism
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurons/*chemistry
MH  - *Nucleic Acid Hybridization
MH  - Oligonucleotide Probes
MH  - RNA, Messenger/*analysis/genetics
MH  - Rats
MH  - Swine
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1016/0014-4886(90)90068-4 [doi]
PST - ppublish
SO  - Exp Neurol. 1990 Aug;109(2):141-52. doi: 10.1016/0014-4886(90)90068-4.