PMID- 23795942
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20130930
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 76
IP  - 1
DP  - 2013 Oct
TI  - Identification and characterization of functional centromeres of the common bean.
PG  - 47-60
LID - 10.1111/tpj.12269 [doi]
AB  - In higher eukaryotes, centromeres are typically composed of megabase-sized arrays 
      of satellite repeats that evolve rapidly and homogenize within a species' genome. 
      Despite the importance of centromeres, our knowledge is limited to a few model 
      species. We conducted a comprehensive analysis of common bean (Phaseolus 
      vulgaris) centromeric satellite DNA using genomic data, fluorescence in situ 
      hybridization (FISH), immunofluorescence and chromatin immunoprecipitation 
      (ChIP). Two unrelated centromere-specific satellite repeats, CentPv1 and CentPv2, 
      and the common bean centromere-specific histone H3 (PvCENH3) were identified. 
      FISH showed that CentPv1 and CentPv2 are predominantly located at subsets of 
      eight and three centromeres, respectively. Immunofluorescence- and ChIP-based 
      assays demonstrated the functional significance of CentPv1 and CentPv2 at 
      centromeres. Genomic analysis revealed several interesting features of CentPv1 
      and CentPv2: (i) CentPv1 is organized into an higher-order repeat structure, 
      named Nazca, of 528 bp, whereas CentPv2 is composed of tandemly organized 
      monomers; (ii) CentPv1 and CentPv2 have undergone chromosome-specific 
      homogenization; and (iii) CentPv1 and CentPv2 are not likely to be commingled in 
      the genome. These findings suggest that two distinct sets of centromere sequences 
      have evolved independently within the common bean genome, and provide insight 
      into centromere satellite evolution.
CI  - (c) 2013 The Authors The Plant Journal (c) 2013 John Wiley & Sons Ltd.
FAU - Iwata, Aiko
AU  - Iwata A
AD  - Center for Applied Genetic Technologies and Institute for Plant Breeding 
      Genetics, and Genomics, University of Georgia, Athens, GA, 30602, USA.
FAU - Tek, Ahmet L
AU  - Tek AL
FAU - Richard, Manon M S
AU  - Richard MM
FAU - Abernathy, Brian
AU  - Abernathy B
FAU - Fonseca, Artur
AU  - Fonseca A
FAU - Schmutz, Jeremy
AU  - Schmutz J
FAU - Chen, Nicolas W G
AU  - Chen NW
FAU - Thareau, Vincent
AU  - Thareau V
FAU - Magdelenat, Ghislaine
AU  - Magdelenat G
FAU - Li, Yupeng
AU  - Li Y
FAU - Murata, Minoru
AU  - Murata M
FAU - Pedrosa-Harand, Andrea
AU  - Pedrosa-Harand A
FAU - Geffroy, Valerie
AU  - Geffroy V
FAU - Nagaki, Kiyotaka
AU  - Nagaki K
FAU - Jackson, Scott A
AU  - Jackson SA
LA  - eng
SI  - GENBANK/KC491791
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130805
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Plant)
RN  - 0 (Histones)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Base Sequence
MH  - *Centromere/genetics/metabolism
MH  - DNA, Complementary/chemistry/genetics
MH  - DNA, Plant/chemistry/genetics
MH  - *Evolution, Molecular
MH  - *Fabaceae/genetics/metabolism
MH  - Histones/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - Plant Proteins/genetics/metabolism
MH  - Species Specificity
OTO - NOTNLM
OT  - Phaseolus vulgaris
OT  - centromere
OT  - centromere evolution
OT  - centromere-specific histone H3
OT  - chromosome-specific homogenization
OT  - higher-order repeat structure
OT  - satellite repeats
EDAT- 2013/06/26 06:00
MHDA- 2014/05/07 06:00
CRDT- 2013/06/26 06:00
PHST- 2013/03/02 00:00 [received]
PHST- 2013/06/15 00:00 [revised]
PHST- 2013/06/20 00:00 [accepted]
PHST- 2013/06/26 06:00 [entrez]
PHST- 2013/06/26 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - 10.1111/tpj.12269 [doi]
PST - ppublish
SO  - Plant J. 2013 Oct;76(1):47-60. doi: 10.1111/tpj.12269. Epub 2013 Aug 5.