PMID- 23800576
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20231213
IS  - 1943-7811 (Electronic)
IS  - 1525-1578 (Linking)
VI  - 15
IP  - 5
DP  - 2013 Sep
TI  - A genome-wide high-resolution array-CGH analysis of cutaneous melanoma and 
      comparison of array-CGH to FISH in diagnostic evaluation.
PG  - 581-91
LID - S1525-1578(13)00092-5 [pii]
LID - 10.1016/j.jmoldx.2013.04.001 [doi]
AB  - Benign melanocytic nevi and cutaneous melanomas can be difficult to differentiate 
      by means of routine microscopic analysis. Recent evidence has suggested that 
      cytogenomic analysis may be a useful diagnostic method for evaluation of 
      melanocytic proliferations. We investigated the array-based comparative genomic 
      hybridization (aCGH) platform for DNA copy number analysis of formalin-fixed, 
      paraffin-embedded (FFPE) tissues in melanocytic tumors and compared aCGH analysis 
      with fluorescence in situ hybridization (FISH) assays in diagnosis of melanoma. 
      aCGH findings and FISH results were interpreted independently in a blinded 
      fashion. Positive findings were not noted in any benign nevi at aCGH analysis, 
      whereas substantial unbalanced genomic aberrations were revealed in 92% of 
      melanomas. Positive results were obtained in 72% of melanomas via the four-probe 
      FISH assay (RREB1/MYB/CEP6/CCND1). A few additional FISH studies were performed 
      to verify some aCGH findings of focal amplification of oncogenes and homozygous 
      deletion of tumor suppressor genes. The overall concordance in aberrations 
      detected using the two methods was 90%. Most discrepancies were due to a minor 
      abnormal clone identified via FISH that was below analytical sensitivity of the 
      FFPE aCGH test. Our study demonstrated that copy number analysis of FFPE tumor 
      samples via aCGH is a robust and reliable method in diagnosis of melanoma and 
      that aCGH and FISH tests should be used as complementary methods to improve the 
      accuracy of genetic evaluation of melanocytic tumors.
CI  - Copyright (c) 2013 American Society for Investigative Pathology and the Association 
      for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
FAU - Wang, Lu
AU  - Wang L
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New 
      York, USA. wangL1@mskcc.org
FAU - Rao, Mamta
AU  - Rao M
FAU - Fang, Yuqiang
AU  - Fang Y
FAU - Hameed, Meera
AU  - Hameed M
FAU - Viale, Agnes
AU  - Viale A
FAU - Busam, Klaus
AU  - Busam K
FAU - Jhanwar, Suresh C
AU  - Jhanwar SC
LA  - eng
PT  - Journal Article
DEP - 20130622
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
SB  - IM
MH  - Algorithms
MH  - *Comparative Genomic Hybridization/methods/standards
MH  - Genetic Variation
MH  - Genome-Wide Association Study/methods/standards
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods/standards
MH  - Melanoma/*diagnosis/genetics/pathology
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms
MH  - Melanoma, Cutaneous Malignant
EDAT- 2013/06/27 06:00
MHDA- 2014/04/09 06:00
CRDT- 2013/06/27 06:00
PHST- 2012/10/18 00:00 [received]
PHST- 2013/02/28 00:00 [revised]
PHST- 2013/04/02 00:00 [accepted]
PHST- 2013/06/27 06:00 [entrez]
PHST- 2013/06/27 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
AID - S1525-1578(13)00092-5 [pii]
AID - 10.1016/j.jmoldx.2013.04.001 [doi]
PST - ppublish
SO  - J Mol Diagn. 2013 Sep;15(5):581-91. doi: 10.1016/j.jmoldx.2013.04.001. Epub 2013 
      Jun 22.