PMID- 23800958
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20211021
IS  - 1348-4400 (Electronic)
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 59
IP  - 5
DP  - 2013 Oct
TI  - Regulation of innate immune function in bovine oviduct epithelial cells in 
      culture: the homeostatic role of epithelial cells in balancing Th1/Th2 response.
PG  - 470-8
AB  - This study aimed to investigate the role of epithelial cells in regulating innate 
      immunity in bovine oviduct epithelial cell (BOEC) culture. We studied the effect 
      of Escherichia coli lipopolysaccharide (LPS) and its interaction with ovarian 
      steroids, estradiol (E2) and progesterone (P4), and luteinizing hormone (LH) at 
      concentrations observed during the preovulatory period on immune responses in 
      BOEC culture. Immunohistochemistry of oviduct tissue showed intensive expression 
      of Toll-like receptor-4 (TLR-4) and TLR-2 in epithelial cells. A dose of 10 ng/ml 
      LPS stimulated TLR-4, cyclooxygenase-2 (COX-2), nuclear factor kappa B inhibitor 
      A (NFKBIA), interleukin 1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) 
      expression, indicating an early pro-inflammatory response. A dose of 100 ng/ml 
      LPS did not induce expression of these genes but stimulated TLR-2, IL-10,IL-4 and 
      microsomal prostaglandin E synthase-1 (mPGES-1) expression and PGE2 secretion, 
      indicating an anti-inflammatory response. Ovarian steroids and LH completely 
      block LPS (10 ng/ml)-induced TLR-4, IL-1beta and TNF-alpha expression as well as LPS 
      (100 ng/ml)-induced TLR-2 expression. Taken together, this study suggests the 
      existence of an early signaling system to respond to infection in the BOEC. In 
      addition, ovarian steroids and LH may play a critical role in inducing 
      homeostasis and in controlling hyperactive pro-inflammatory responses detrimental 
      to epithelial cells, sperm and the embryo.
FAU - Kowsar, Rasoul
AU  - Kowsar R
AD  - Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and 
      Veterinary Medicine, Hokkaido 080-8555, Japan.
FAU - Hambruch, Nina
AU  - Hambruch N
FAU - Liu, Jinghui
AU  - Liu J
FAU - Shimizu, Takashi
AU  - Shimizu T
FAU - Pfarrer, Christiane
AU  - Pfarrer C
FAU - Miyamoto, Akio
AU  - Miyamoto A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20130621
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (lipopolysaccharide, E coli O55-B5)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Abattoirs
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Epithelium/drug effects/*immunology/metabolism
MH  - Estradiol/metabolism
MH  - Fallopian Tubes/cytology/drug effects/*immunology/metabolism
MH  - Female
MH  - *Homeostasis/drug effects
MH  - *Immunity, Innate/drug effects
MH  - *Immunity, Mucosal/drug effects
MH  - Immunomodulation/drug effects
MH  - Lipopolysaccharides/antagonists & inhibitors/toxicity
MH  - Luteinizing Hormone/metabolism
MH  - *Models, Biological
MH  - Progesterone/metabolism
MH  - *Th1-Th2 Balance/drug effects
MH  - Toll-Like Receptor 2/biosynthesis/genetics/metabolism
MH  - Toll-Like Receptor 4/biosynthesis/genetics/metabolism
MH  - Up-Regulation
PMC - PMC3934114
EDAT- 2013/06/27 06:00
MHDA- 2014/06/17 06:00
PMCR- 2013/10/01
CRDT- 2013/06/27 06:00
PHST- 2013/06/27 06:00 [entrez]
PHST- 2013/06/27 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - DN/JST.JSTAGE/jrd/2013-036 [pii]
AID - 2013-036 [pii]
AID - 10.1262/jrd.2013-036 [doi]
PST - ppublish
SO  - J Reprod Dev. 2013 Oct;59(5):470-8. doi: 10.1262/jrd.2013-036. Epub 2013 Jun 21.