PMID- 23801500
OWN - NLM
STAT- MEDLINE
DCOM- 20140501
LR  - 20211021
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 24
IP  - 10
DP  - 2013 Oct
TI  - Ultrathin sP(EO-stat-PO) hydrogel coatings are biocompatible and preserve 
      functionality of surface bound growth factors in vivo.
PG  - 2417-27
LID - 10.1007/s10856-013-4984-4 [doi]
AB  - Hydrogel coatings prepared from reactive star shaped polyethylene oxide based 
      prepolymers (NCO-sP(EO-stat-PO)) minimize unspecific protein adsorption in vitro, 
      while proteins immobilized on NCO-sP(EO-stat-PO) coatings retain their structure 
      and biological function. The aim of the present study was to assess 
      biocompatibility and the effect on early osseointegrative properties of a 
      NCO-sP(EO-stat-PO) coating with additional RGD-peptides and augmentation with 
      bone morphogenetic protein-4 (BMP) used on a medical grade high-density 
      polyethylene (HDPE) base under in vivo circumstances. For testing of 
      biocompatibility dishes with large amounts of bulk NCO-sP(EO-stat-PO) were 
      implanted subcutaneously into 14 Wistar rats. In a second set-up 
      functionalization of implants with ultrathin surface layers by coating 
      ammonia-plasma treated HDPE with NCO-sP(EO-stat-PO), functionalization with 
      linear RGD-peptides, and augmentation with RGD and BMP-4 was analyzed. Therefore, 
      implants were placed subcutaneously in the paravertebral tissue and 
      transcortically in the distal femur of another 14 Wistar rats. Both tests 
      revealed no signs of enhanced inflammation of the surrounding tissue analyzed by 
      CD68, IL-1ss-/TNF-alpha-antibody staining, nor systemic toxic reactions according to 
      histological analysis of various organs. The mean thickness of the fibrous tissue 
      surrounding the femoral implants was highest in native HDPE-implants and tended 
      to be lower in all NCO-sP(EO-stat-PO) modified implants. Micro-CT analysis 
      revealed a significant increase of peri-implant bone volume in RGD/BMP-4 coated 
      samples. These results demonstrate that even very low amounts of surface bound 
      growth factors do have significant effects when immobilized in an environment 
      that retains their biological function. Hence, NCO-sP(EO-stat-PO)-coatings could 
      offer an attractive platform to improve integration of orthopedic implants.
FAU - Neuerburg, Carl
AU  - Neuerburg C
AD  - Department of Orthopaedics, University of Ulm, Ulm, Germany.
FAU - Recknagel, Stefan
AU  - Recknagel S
FAU - Fiedler, Jorg
AU  - Fiedler J
FAU - Groll, Jurgen
AU  - Groll J
FAU - Moeller, Martin
AU  - Moeller M
FAU - Bruellhoff, Kristina
AU  - Bruellhoff K
FAU - Reichel, Heiko
AU  - Reichel H
FAU - Ignatius, Anita
AU  - Ignatius A
FAU - Brenner, Rolf E
AU  - Brenner RE
LA  - eng
PT  - Journal Article
DEP - 20130626
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Hydrogels)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Oligopeptides)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Bone Morphogenetic Protein 4/chemistry
MH  - Cell Adhesion
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Femur/pathology
MH  - Hydrogels/*chemistry
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Oligopeptides/*chemistry
MH  - Osseointegration
MH  - Polyethylene Glycols/*chemistry
MH  - Prostheses and Implants
MH  - Rats
MH  - Rats, Wistar
MH  - Surface Properties
MH  - Time Factors
MH  - X-Ray Microtomography
EDAT- 2013/06/27 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/06/27 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/06/11 00:00 [accepted]
PHST- 2013/06/27 06:00 [entrez]
PHST- 2013/06/27 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.1007/s10856-013-4984-4 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2013 Oct;24(10):2417-27. doi: 10.1007/s10856-013-4984-4. 
      Epub 2013 Jun 26.