PMID- 23801650
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20211021
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 121
IP  - 10
DP  - 2013 Oct
TI  - Evaluation of urovysion and cytology for bladder cancer detection: a study of 
      1835 paired urine samples with clinical and histologic correlation.
PG  - 591-7
LID - 10.1002/cncy.21327 [doi]
AB  - BACKGROUND: Urine cytology has been used for screening of bladder cancer but has 
      been limited by its low sensitivity. UroVysion is a multiprobe fluorescence in 
      situ hybridization (FISH) assay that detects common chromosome abnormalities in 
      bladder cancers. For this study, the authors evaluated the effectiveness of 
      multiprobe FISH and urine cytology in detecting urothelial cell carcinoma (UCC) 
      in the same urine sample. METHODS: In total, 1835 cases with the following 
      criteria were selected: valid results from both the multiprobe FISH assay and 
      urine cytology in the same urine sample, histologic and/or cystoscopic follow-up 
      within 4 months of the original tests, or at least 3 years of clinical follow-up 
      information. The results of FISH and cytology were correlated with clinical 
      outcomes derived from a combination of histologic, cystoscopic, and clinical 
      follow-up information. RESULTS: Of 1835 cases, 1045 cases were from patients 
      undergoing surveillance of recurrent UCC, and 790 were for hematuria. The overall 
      sensitivity, specificity, positive predictive value, and negative predictive 
      value in detecting UCC were 61.9%, 89.7%, 53.9%, and 92.4%, respectively, for 
      FISH and 29.1%, 96.9%, 64.4%, and 87.5%, respectively, for cytology. The 
      performance of both FISH and cytology generally was better in the surveillance 
      population and in samples with high-grade UCC. In 95 of 296 cases with atypical 
      cytology that were proven to have UCC, 61 cases, mostly high-grade UCC, were 
      positive using the multiprobe FISH assay. CONCLUSIONS: The UroVysion multiprobe 
      FISH assay was more sensitive than urine cytology in detecting UCC, but it 
      produced more false-positive results. The current data suggest that the use of 
      FISH as a reflex test after an equivocal cytologic diagnosis may play an 
      effective role in detecting UCC.
CI  - (c) 2013 American Cancer Society.
FAU - Dimashkieh, Haythem
AU  - Dimashkieh H
AD  - Department of Pathology and Laboratory Medicine, Medical University of South 
      Carolina, Charleston, South Carolina.
FAU - Wolff, Daynna J
AU  - Wolff DJ
FAU - Smith, T Michael
AU  - Smith TM
FAU - Houser, Patricia M
AU  - Houser PM
FAU - Nietert, Paul J
AU  - Nietert PJ
FAU - Yang, Jack
AU  - Yang J
LA  - eng
GR  - UL1 TR000062/TR/NCATS NIH HHS/United States
GR  - UL1TR000062/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130625
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Chromosome Aberrations
MH  - Cystoscopy
MH  - *Cytodiagnosis
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Grading
MH  - Neoplasm Recurrence, Local/*diagnosis
MH  - Prognosis
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/*diagnosis
MH  - Urine/*chemistry/cytology
PMC - PMC3800248
MID - NIHMS488414
OTO - NOTNLM
OT  - UroVysion
OT  - bladder cancers
OT  - urine cytology
OT  - urothelial cell carcinoma
EDAT- 2013/06/27 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/10/01
CRDT- 2013/06/27 06:00
PHST- 2013/03/14 00:00 [received]
PHST- 2013/05/10 00:00 [revised]
PHST- 2013/05/15 00:00 [accepted]
PHST- 2013/06/27 06:00 [entrez]
PHST- 2013/06/27 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 10.1002/cncy.21327 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2013 Oct;121(10):591-7. doi: 10.1002/cncy.21327. Epub 2013 Jun 
      25.