PMID- 23802415
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20190923
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 9
IP  - 5
DP  - 2013 May
TI  - Analysis of nanoscale protein film consisting of lactoferrin/11-MUA bilayers for 
      bioelectronic device.
PG  - 849-55
AB  - We fabricated and analyzed a nanoscale biofilm of human lactoferrin making use of 
      11-mercapto-undecanoic acid (11-MUA) as chemical linker. The fabrication of the 
      bimolecular/organic hetero monolayer (lactoferrin/11-MUA) on gold substrate was 
      confirmed with Raman spectroscopy. Cyclic voltammetry (CV) was carried out to 
      observe the electrochemical properties of the nanoscaled biofilm under various pH 
      conditions and at different time intervals. The well-defined redox properties 
      were observed, even in certain harsh pH conditions and after a long time, proving 
      the stabilities of this biofilm. Atomic force microscopy (AFM) was further 
      employed to confirm the retention time by investigating the morphology variety of 
      the biofilm over time. All these results proved that, the proposed nanoscaled 
      thin film composed of lactoferrin and 11-MUA is a powerful alternative for making 
      bioelectronics devices.
FAU - Chen, Qi
AU  - Chen Q
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, 35 
      Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
FAU - Lee, Taek
AU  - Lee T
FAU - Kumar, Ajay Yagati
AU  - Kumar AY
FAU - Min, Junhong
AU  - Min J
FAU - Choi, Jeong-Woo
AU  - Choi JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (11-mercaptoundecanoic acid)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Sulfhydryl Compounds)
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Bioengineering/methods
MH  - *Electrical Equipment and Supplies
MH  - Electrochemical Techniques
MH  - Fatty Acids/*chemistry
MH  - Humans
MH  - Lactoferrin/*chemistry
MH  - Lipid Bilayers/*analysis/chemistry
MH  - Microscopy, Atomic Force
MH  - Models, Biological
MH  - Multiprotein Complexes/*analysis/chemistry
MH  - Nanostructures/chemistry
MH  - Oxidation-Reduction
MH  - Spectrum Analysis, Raman
MH  - Sulfhydryl Compounds/*chemistry
MH  - Surface Properties
EDAT- 2013/06/28 06:00
MHDA- 2013/08/06 06:00
CRDT- 2013/06/28 06:00
PHST- 2013/06/28 06:00 [entrez]
PHST- 2013/06/28 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
AID - 10.1166/jbn.2013.1496 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2013 May;9(5):849-55. doi: 10.1166/jbn.2013.1496.