PMID- 23803103
OWN - NLM
STAT- MEDLINE
DCOM- 20141014
LR  - 20170308
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 14
IP  - 5
DP  - 2013
TI  - In vitro antitumor properties of an isolate from leaves of Cassia alata L.
PG  - 3191-6
AB  - Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a 
      variety of diseases, were evaluated for their potential antitumor properties in 
      vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on 
      five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, 
      SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal 
      carcinoma, and A549, a non- small cell lung adenocarcinoma. Hexane extracts 
      showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent 
      manner. This observation was confirmed by morphological investigation using light 
      microscopy. Further bioassay-directed fractionation of the cytotoxic extract led 
      to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further 
      evaluated using MTT assay and morphological and biochemical investigations, which 
      likewise showed selectivity to MCF-7, T24, and Col 2 cells with IC50 values of 
      16, 17, and 17 mug/ml, respectively. Isolate f6l, however, showed no cytotoxicity 
      towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical 
      investigation using DAPI staining and biochemical investigation using terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method 
      used to detect DNA fragmentation-together with caspase assay, demonstrated 
      apoptotic cell death. Spectral characterization of isolate f6l revealed that it 
      contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile 
      and its mode of action, f6l might represent a new promising compound with 
      potential for development as an anticancer drug with low or no toxicity to 
      non-cancer cells used in this study.
FAU - Olarte, Elizabeth Iglesias
AU  - Olarte EI
AD  - Institute of Biology, College of Science, University of the Philippines, Diliman, 
      Quezon City, Philippines. liziglesias.olarte@gmail.com
FAU - Herrera, Annabelle Aliga
AU  - Herrera AA
FAU - Villasenor, Irene Manese
AU  - Villasenor IM
FAU - Jacinto, Sonia Donaldo
AU  - Jacinto SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Plant Extracts)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - CHO Cells
MH  - Caspase 3/metabolism
MH  - Cassia/*chemistry
MH  - Cell Proliferation/*drug effects
MH  - Cricetinae
MH  - Cricetulus
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Neoplasms/*drug therapy/enzymology/pathology
MH  - *Phytotherapy
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/*chemistry
MH  - Tumor Cells, Cultured
EDAT- 2013/06/28 06:00
MHDA- 2014/10/15 06:00
CRDT- 2013/06/28 06:00
PHST- 2013/06/28 06:00 [entrez]
PHST- 2013/06/28 06:00 [pubmed]
PHST- 2014/10/15 06:00 [medline]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2013;14(5):3191-6.