PMID- 23803215
OWN - NLM
STAT- MEDLINE
DCOM- 20140724
LR  - 20181203
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 33
IP  - 6
DP  - 2013 Jun
TI  - [Effect of exendin-4 on monocyte chemoattractant protein-1 expression in cultured 
      rat glomerular mesangial cells induced by tumor necrosis factor-alpha in vitro].
PG  - 930-3
AB  - OBJECTIVE: To investigate the effect of exendin-4 on the expression of monocyte 
      chemoattractant protein-1 (MCP-1) and fibronectin (FN) in rat glomerular 
      mesangial cells in vitro. METHODS: Rat glomerular mesangial cells were divided 
      into 5 groups, namely control group, tumor necrosis factor-alpha (TNF-alpha) group (10 
      ng/ml), TNF-alpha (10 ng/ml)+E1 (1 nmol/L exendin-4) group, TNF-alpha (10 ng/ml)+E5 (5 
      nmol/L exendin-4) group, and TNF-alpha (10 ng/ml)+E10 (10 nmol/L exendin-4) group. 
      After cultured 24 h or 48 h, RNA were extracted to determine the expression of 
      MCP-1 with real-time PCR, the supernatant were collected to determine the 
      expression of MCP-1 and FN with ELISA. RESULTS: Compared with control group, the 
      cells treated with TNF-alpha for 24 h showed significantly increase the expression of 
      MCP-1 and FN (P<0.01), exendin-4 significantly reduced the expression of MCP-1 
      and FN in TNF-alpha+E5 group and TNF-alpha+E10 group (P<0.05). After 48h incubation, the 
      expression of MCP-1 and FN increased significantly in TNF-alpha group (P<0.01), which 
      was lowered by exendin-4 in TNF-alpha+E1,TNF-alpha+E5 and TNF-alpha+E10 groups (P<0.05). 
      CONCLUSION: Exendin-4 has an intrinsic capability to concentration- and 
      time-dependently inhibit TNF-alpha-induced expression of MCP-1 and FN in rat 
      mesangial cells, suggesting the beneficial effect of exendin-4 in preventing and 
      treating diabetic nephropathy.
FAU - Jiang, Yingjuan
AU  - Jiang Y
AD  - Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical 
      University, Guangzhou 510515, China. yingjuanjiang@126.com
FAU - Xue, Yaoming
AU  - Xue Y
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Zhang, Yanfei
AU  - Zhang Y
FAU - Yuan, Yuan
AU  - Yuan Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Peptides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Venoms)
RN  - 9P1872D4OL (Exenatide)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Diabetic Nephropathies/*metabolism
MH  - Exenatide
MH  - Glomerular Mesangium/cytology
MH  - Mesangial Cells/drug effects/*metabolism
MH  - Peptides/*pharmacology
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Venoms/*pharmacology
EDAT- 2013/06/28 06:00
MHDA- 2014/07/25 06:00
CRDT- 2013/06/28 06:00
PHST- 2013/06/28 06:00 [entrez]
PHST- 2013/06/28 06:00 [pubmed]
PHST- 2014/07/25 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jun;33(6):930-3.