PMID- 23804579
OWN - NLM
STAT- MEDLINE
DCOM- 20141103
LR  - 20140305
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Linking)
VI  - 22
IP  - 3
DP  - 2014 Mar
TI  - Obesity rather than regional fat depots marks the metabolomic pattern of adipose 
      tissue: an untargeted metabolomic approach.
PG  - 698-704
LID - 10.1002/oby.20541 [doi]
AB  - OBJECTIVE: This study compares the patterns of visceral (VIS) and subcutaneous 
      (SC) adipose tissue (AT)-derived metabolites from non-obese (BMI 24-26 kg/m2) and 
      obese subjects (BMI > 40 kg/m2) with no major metabolic risk factors other than 
      BMI. METHODS: SC- and VIS- AT obtained from obese (Ob) and non-obese (NOb) 
      subjects during surgery were incubated to obtain their metabolites. Differences 
      related to obesity or anatomical provenances of AT were assessed using an 
      untargeted metabolomics approach based on gas chromatography-mass spectrometry. 
      RESULTS: The overall effect of obesity on the metabolite profile resulted more 
      remarkable than the effect of regional AT. Only the depletion of 2-ketoisocaproic 
      (2-KIC) acid reached statistical significance for the SC-AT alone, although it 
      was observed in both depots. Obesity induced more significant changes in several 
      amino acids levels of the VIS-AT metabolites. On the one hand, higher released 
      levels of glutamine and alanine were detected in the VIS- obese AT, whereas on 
      the other, the VIS- obese AT presented a diminished uptake of essential amino 
      acids (methionine, threonine, lysine), BCAAs, leucine, and serine. CONCLUSION: 
      This study shows that obesity markedly affects the amino acid metabolic signature 
      of the AT before the clinical onset of other significant metabolic alterations 
      aside from BMI.
CI  - Copyright (c) 2013 The Obesity Society.
FAU - Hanzu, F A
AU  - Hanzu FA
AD  - Department of Endocrinology and Nutrition, Diabetes and Obesity Research 
      Laboratory, Hospital Clinic de Barcelona, Institut d'Investigacions Biomediques 
      August Pi i Sunyer (IDIBAPS), Spain; CIBER de Diabetes y Enfermedades Metabolicas 
      Asociadas (CIBERDEM), Spain.
FAU - Vinaixa, M
AU  - Vinaixa M
FAU - Papageorgiou, A
AU  - Papageorgiou A
FAU - Parrizas, M
AU  - Parrizas M
FAU - Correig, X
AU  - Correig X
FAU - Delgado, S
AU  - Delgado S
FAU - Carmona, F
AU  - Carmona F
FAU - Samino, S
AU  - Samino S
FAU - Vidal, J
AU  - Vidal J
FAU - Gomis, R
AU  - Gomis R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130918
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Amino Acids)
SB  - IM
MH  - Adult
MH  - Amino Acids/metabolism
MH  - *Body Fat Distribution
MH  - Body Mass Index
MH  - Case-Control Studies
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Intra-Abdominal Fat/*metabolism
MH  - Male
MH  - *Metabolome
MH  - Middle Aged
MH  - Obesity, Morbid/*metabolism
MH  - Risk Factors
MH  - Subcutaneous Fat/metabolism
EDAT- 2013/06/28 06:00
MHDA- 2014/11/05 06:00
CRDT- 2013/06/28 06:00
PHST- 2012/12/17 00:00 [received]
PHST- 2013/05/25 00:00 [revised]
PHST- 2013/05/26 00:00 [accepted]
PHST- 2013/06/28 06:00 [entrez]
PHST- 2013/06/28 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - 10.1002/oby.20541 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2014 Mar;22(3):698-704. doi: 10.1002/oby.20541. Epub 
      2013 Sep 18.