PMID- 23806036
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20190402
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 56
IP  - 4
DP  - 2013 Aug 31
TI  - Implication of cerebral dopamine-beta hydroxylase for cardiovascular and mood 
      regulation in rats.
PG  - 209-18
AB  - The essentiality of the role of norepinephrine (NE) in the central nervous system 
      has recently been reconsidered. NE exerts many effects and mediates a number of 
      functions in living organisms. Dopamine-beta-hydroxylase (DBH) is the crucial 
      enzyme for NE and epinephrine biosynthesis. Removal of this enzyme causes 
      deficient NE at sympathetic terminals characterized by orthostatic hypotension in 
      humans. The hypothesis tested in this study was that NE deficiency in the central 
      nervous system caused autonomic failure in cardiovascular regulation. The 
      immunotoxin anti-DBH-saporin (DSAP) was used to examine the putative role of 
      cerebral NE. Male Sprague-Dawley rats were injected, intracerebroventricularly 
      (icv), with DSAP and cardiovascular reactivity, as well as behavioral variables 
      in the open-field locomotion test (OLT), sucrose intake test (SIT) and forced 
      swim test (FST), were monitored for changes. The results indicated that treatment 
      with DSAP caused significant reductions in spontaneous blood pressure (BP) and 
      heart rate (HR), and a decrease in the rearing position on the OLT, in the same 
      group of rats. In addition, a significant increase in mobility with low 
      concurrent immobility frequencies was observed on the FST. However, there was no 
      variation on the SIT. In conclusion, a deficiency in the cerebral DBH might 
      dysregulate the autonomic outflows and, thus, leads to lower BP and HR. However, 
      there was no mood change such as despair or anhedonia observed in the 
      experiments.
FAU - Chang, Shang-Tang
AU  - Chang ST
AD  - Medical Research Center, Cardinal Tien Hospital, Taipei 23148, Taiwan, Republic 
      of China.
FAU - Liu, Yia-Ping
AU  - Liu YP
AD  - Department of Physiology and Biophysics, National Defense Medical Center, Taipei 
      11490, Taiwan, Republic of China.
FAU - Huang, Chuen-Lin
AU  - Huang CL
AD  - Medical Research Center, Cardinal Tien Hospital, Taipei 23148, Taiwan, Republic 
      of China.
FAU - Wang, Pei-Ying
AU  - Wang PY
AD  - Department of Physiology and Biophysics, National Defense Medical Center, Taipei 
      11490, Taiwan, Republic of China.
FAU - Tung, Che-Se
AU  - Tung CS
AD  - Department of Physiology and Biophysics, National Defense Medical Center, Taipei 
      11490, Taiwan, Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
SB  - IM
EIN - Chin J Physiol. 2013 Oct 31;56(5):306
MH  - *Affect
MH  - Animals
MH  - *Blood Pressure
MH  - Brain/*enzymology
MH  - Dopamine beta-Hydroxylase/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - *Heart Rate
MH  - Male
MH  - Motor Activity
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2013/06/29 06:00
MHDA- 2014/05/07 06:00
CRDT- 2013/06/29 06:00
PHST- 2013/06/29 06:00 [entrez]
PHST- 2013/06/29 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - CJP.2013.BAB103 [pii]
AID - 10.4077/CJP.2013.BAB103 [doi]
PST - ppublish
SO  - Chin J Physiol. 2013 Aug 31;56(4):209-18. doi: 10.4077/CJP.2013.BAB103.