PMID- 23808658
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20181202
IS  - 1543-8392 (Electronic)
IS  - 1543-8384 (Linking)
VI  - 10
IP  - 8
DP  - 2013 Aug 5
TI  - Epidermal stem cells manipulated by pDNA-VEGF165/CYD-PEI nanoparticles loaded 
      gelatin/beta-TCP matrix as a therapeutic agent and gene delivery vehicle for wound 
      healing.
PG  - 3090-102
LID - 10.1021/mp400162k [doi]
AB  - The success of gene therapy largely relies on a safe and effective gene delivery 
      system. The objective of this study is to design a highly efficient system for 
      the transfection of epidermal stem cells (ESCs) and investigate the transfected 
      ESCs (TESCs) as a therapeutic agent and gene delivery reservoir for wound 
      treatment. As a nonviral vector, beta-cyclodextrin-linked polyethylenimines 
      (CYD-PEI) was synthesized by linking beta-cyclodextrin with polyethylenimines (600 
      Da). Gelatin scaffold incorporating beta-tricalcium phosphate (beta-TCP) was utilized 
      as a substrate for the culture and transfection of ESCs. With the 
      CYD-PEI/pDNA-VEGF165 polyplexes incorporated gelatin/beta-TCP scaffold based 3D 
      transfection system, prolonged VEGF expression with a higher level was obtained 
      at day 7 in ESCs than those in two-dimensional plates. Topical application of the 
      TESCs significantly accelerated the skin re-epithelization, dermal collagen 
      synthesis, and hair follicle regeneration. It also exhibited a potential in scar 
      inhibition by regulating the distribution of different types of collagen. In 
      contrast to ESCs, an additive capacity in stimulating angiogenesis at the wound 
      site was observed in the TESCs. The present study provides a basis for the TESCs 
      as a promising therapeutic agent and gene delivery reservoir for wound therapy.
FAU - Peng, Li-Hua
AU  - Peng LH
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University , Hangzhou, Zhejiang, 310058, PR China.
FAU - Wei, Wei
AU  - Wei W
FAU - Qi, Xiao-Tian
AU  - Qi XT
FAU - Shan, Ying-Hui
AU  - Shan YH
FAU - Zhang, Fang-Jun
AU  - Zhang FJ
FAU - Chen, Xi
AU  - Chen X
FAU - Zhu, Qian-Ying
AU  - Zhu QY
FAU - Yu, Lian
AU  - Yu L
FAU - Liang, Wen-Quan
AU  - Liang WQ
FAU - Gao, Jian-Qing
AU  - Gao JQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130628
PL  - United States
TA  - Mol Pharm
JT  - Molecular pharmaceutics
JID - 101197791
RN  - 0 (Calcium Phosphates)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (beta-tricalcium phosphate)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 9000-70-8 (Gelatin)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - JV039JZZ3A (betadex)
SB  - IM
MH  - Animals
MH  - Calcium Phosphates/*chemistry
MH  - Cells, Cultured
MH  - *Epidermal Cells
MH  - Gelatin/*chemistry
MH  - Gene Transfer Techniques
MH  - Genetic Therapy
MH  - Nanoparticles/chemistry
MH  - Polyethyleneimine/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/cytology/*metabolism/physiology
MH  - Vascular Endothelial Growth Factor A/*genetics
MH  - Wound Healing/physiology/*radiation effects
MH  - beta-Cyclodextrins/*chemistry
EDAT- 2013/07/03 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/07/02 06:00
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - 10.1021/mp400162k [doi]
PST - ppublish
SO  - Mol Pharm. 2013 Aug 5;10(8):3090-102. doi: 10.1021/mp400162k. Epub 2013 Jun 28.