PMID- 23809529
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20220408
IS  - 1079-0268 (Print)
IS  - 2045-7723 (Electronic)
IS  - 1079-0268 (Linking)
VI  - 36
IP  - 2
DP  - 2013 Mar
TI  - Sperm chromatin condensation, DNA integrity, and apoptosis in men with spinal 
      cord injury.
PG  - 140-6
LID - 10.1179/2045772312Y.0000000055 [doi]
AB  - OBJECTIVES: To evaluate the effect of cord injury on (1) sperm parameters and (2) 
      DNA chromatin status. DESIGN: Case-control study. SETTING: Data were collected 
      from men referred to Research and Clinical Center for Infertility, Yazd, Iran. 
      PARTICIPANTS: Thirty infertile men with the presence of any level of spinal cord 
      injury (SCI) were compared with 30 healthy donors with definite fertility and 
      normal sperm parameters. INTERVENTIONS: Not applicable. OUTCOME MEASURES: Sperm 
      chromatin integrity was assessed using aniline blue (AB), chromomycin A3 (CMA3), 
      toluidine blue (TB), and acridine orange (AO) assays. The rate of apoptotic 
      spermatozoa was evaluated with terminal deoxynucleotidyl transferase-mediated 
      dUTP nick-end labelling (TUNEL) staining. RESULTS: Sperm concentration, motility, 
      and morphology in men with SCI were significantly decreased compared with control 
      group (P < 0.05). In addition, with regard to cytochemical staining and TUNEL 
      test, the rate of reacted spermatozoa was increased significantly in SCI group 
      when compared with the controls (P < 0.05). The majority of AB, TB, AO, and 
      CMA3-reacted spermatozoa were higher than the "cut-off" value in men with SCI, as 
      were the number of apoptotic spermatozoa stained with TUNEL. CONCLUSION: Results 
      showed that SCI disturbs sperm parameters, nuclear maturity, and DNA integrity of 
      spermatozoa. Therefore, the production of spermatozoa with less condensed 
      chromatin and more apoptotic rate increases after cord injury and this may be one 
      possible cause of infertility following SCI.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Khalili, Mohammad Ali
AU  - Khalili MA
FAU - Vahidi, Serajodin
AU  - Vahidi S
FAU - Ghasemzadeh, Jalal
AU  - Ghasemzadeh J
FAU - Tabibnejad, Nasim
AU  - Tabibnejad N
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Spinal Cord Med
JT  - The journal of spinal cord medicine
JID - 9504452
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Apoptosis/*genetics
MH  - Case-Control Studies
MH  - Chromatin/genetics/*pathology
MH  - DNA/*genetics
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Infertility, Male/etiology/genetics/pathology
MH  - Male
MH  - Spermatozoa/*pathology
MH  - Spinal Cord Injuries/*complications/pathology
PMC - PMC3595962
EDAT- 2013/07/03 06:00
MHDA- 2013/08/09 06:00
PMCR- 2014/03/01
CRDT- 2013/07/02 06:00
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - scm-36-140 [pii]
AID - 10.1179/2045772312Y.0000000055 [doi]
PST - ppublish
SO  - J Spinal Cord Med. 2013 Mar;36(2):140-6. doi: 10.1179/2045772312Y.0000000055.