PMID- 23810682
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20220330
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 116
IP  - 1
DP  - 2014 Jan
TI  - Effects of a novel cell stabilizing reagent on DNA amplification by PCR as 
      compared to traditional stabilizing reagents.
PG  - 55-60
LID - S0065-1281(13)00090-1 [pii]
LID - 10.1016/j.acthis.2013.05.002 [doi]
AB  - Stabilization of nucleated blood cells by cell stabilizing reagent (BCT reagent) 
      present in the Cell-Free DNA BCT blood collection device and consequent 
      prevention of cell-free DNA contamination by cellular DNA during sample storage 
      and shipping have previously been reported. This study was conducted to 
      investigate the effect of this novel cell stabilizing reagent on DNA 
      amplification by PCR as compared to traditional cell stabilizing reagents, 
      formaldehyde and glutaraldehyde. A 787 bp long DNA fragment from human 
      glyceraldehydes-3-phosphate dehydrogenase (GAPDH) gene was amplified by PCR and 
      used as model system. DNA samples and blood samples were treated with BCT 
      reagent, 0.1% formaldehyde or 0.1% glutaraldehyde at room temperature. DNA 
      amplification was studied using conventional and real-time quantitative PCR. 
      Results indicate that exposure of DNA to the BCT reagent for up to 14 days had no 
      effect on DNA amplification by PCR as compared to the untreated control DNA. 
      However, there was statistically significant decrease in DNA amplification in the 
      DNA samples treated with formaldehyde and glutaraldehyde. We conclude that the 
      BCT reagent used in Cell-Free DNA BCT blood collection device to prevent 
      cell-free DNA contamination by cellular DNA had no effect on DNA amplification by 
      PCR.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Das, Kausik
AU  - Das K
AD  - Streck, Inc., 7002 S 109 Street, La Vista, NE 68128, USA. Electronic address: 
      kdas@streck.com.
FAU - Fernando, M Rohan
AU  - Fernando MR
AD  - Streck, Inc., 7002 S 109 Street, La Vista, NE 68128, USA. Electronic address: 
      rfernando@streck.com.
FAU - Basiaga, Sara
AU  - Basiaga S
AD  - Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA. 
      Electronic address: sbasiaga@unl.edu.
FAU - Wigginton, Stephanie M
AU  - Wigginton SM
AD  - Streck, Inc., 7002 S 109 Street, La Vista, NE 68128, USA. Electronic address: 
      swigginton@streck.com.
FAU - Williams, Tom
AU  - Williams T
AD  - Methodist Hospital Laboratories, 8303 Dodge Street, Omaha, NE 68114, USA. 
      Electronic address: Tom.Williams@nmhs.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130628
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Indicators and Reagents)
RN  - 9007-49-2 (DNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Blood Specimen Collection
MH  - DNA/*chemical synthesis/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Humans
MH  - Indicators and Reagents/chemistry
MH  - Leukocytes/physiology
MH  - *Polymerase Chain Reaction
OTO - NOTNLM
OT  - Cell-free DNA BCT reagent
OT  - DNA amplification
OT  - DNA fluorescence
OT  - Formaldehyde free stabilizing reagent
EDAT- 2013/07/03 06:00
MHDA- 2015/05/15 06:00
CRDT- 2013/07/02 06:00
PHST- 2013/04/02 00:00 [received]
PHST- 2013/05/12 00:00 [revised]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
AID - S0065-1281(13)00090-1 [pii]
AID - 10.1016/j.acthis.2013.05.002 [doi]
PST - ppublish
SO  - Acta Histochem. 2014 Jan;116(1):55-60. doi: 10.1016/j.acthis.2013.05.002. Epub 
      2013 Jun 28.