PMID- 23811107
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20190504
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 36
IP  - 2
DP  - 2013 Sep
TI  - T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through 
      oxidative stress.
PG  - 493-500
LID - S1382-6689(13)00076-8 [pii]
LID - 10.1016/j.etap.2013.03.017 [doi]
AB  - OBJECTIVE: To investigate the reproductive toxicity and cytotoxicity of T-2 
      toxin, which is a mycotoxin, and to explore its potential apoptotic induction 
      mechanism. METHODS: ovarian granulosa cells of rats were treated with T-2 toxin 
      (1-100nM) for 24h. The cytotoxicity was assessed with MTT bioassay and apoptotic 
      cells were detected by flow cytometry, and further identified by chromatin 
      condensation and nuclear fragmentation with Hoechst 33258 under microscope; 
      reactive oxygen species (ROS) with DCFH-DA was analyzed by fluorometry; total 
      superoxide dismutase (SOD) was determined by NBT staining method. Glutathione 
      peroxidase (GSHPx) activity was detected by UV colorimetric assay. The activity 
      of catalase (CAT) in granulosa cells was measured by the Aebi method, and 
      malondialdehyde (MDA) content was determined by thiobarbituric acid assay. 
      RESULTS: T-2 toxin dose-dependently inhibited the growth of granulosa cells and 
      resulted in apoptosis in rat granulosa cells. Treatment with T-2 toxin could 
      induce ROS and MDA accumulation in granulosa cells, acompanying with losses of 
      activities of SOD, GSH-Px and CAT, whereas T-2 toxin-induced apoptosis in 
      granulosa cells could be significantly inhibited through the use of antioxidant 
      Trolox. CONCLUSION: These data suggest that T-2 toxin may induce the apoptosis in 
      rat granulosa cells through oxidative stress.
CI  - Copyright (c) 2013. Published by Elsevier B.V.
FAU - Wu, Jing
AU  - Wu J
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 
      410128, PR China.
FAU - Tu, Di
AU  - Tu D
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 
      410128, PR China.
FAU - Yuan, Li-Yun
AU  - Yuan LY
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 
      410128, PR China.
FAU - Yuan, Hui
AU  - Yuan H
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 
      410128, PR China.
FAU - Wen, Li-Xin
AU  - Wen LX
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 
      410128, PR China. Electronic address: sfwlx8015@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20130411
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Antioxidants)
RN  - 0 (Chromans)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - I3FL5NM3MO (T-2 Toxin)
RN  - S18UL9710X (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/*drug effects
MH  - Catalase/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chromans/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Glutathione Peroxidase/metabolism
MH  - Granulosa Cells/*drug effects/metabolism/pathology
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - T-2 Toxin/*toxicity
MH  - Thiobarbituric Acid Reactive Substances/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Granulosa cells
OT  - Oxidative stress
OT  - T-2 toxin
EDAT- 2013/07/03 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/07/02 06:00
PHST- 2012/08/27 00:00 [received]
PHST- 2013/03/21 00:00 [revised]
PHST- 2013/03/22 00:00 [accepted]
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - S1382-6689(13)00076-8 [pii]
AID - 10.1016/j.etap.2013.03.017 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2013 Sep;36(2):493-500. doi: 
      10.1016/j.etap.2013.03.017. Epub 2013 Apr 11.