PMID- 23811547
OWN - NLM
STAT- MEDLINE
DCOM- 20140722
LR  - 20220409
IS  - 1735-367X (Electronic)
IS  - 1735-1383 (Linking)
VI  - 10
IP  - 2
DP  - 2013 Jun
TI  - Interleukin-17 serum levels and TLR4 polymorphisms in ulcerative colitis.
PG  - 83-92
AB  - BACKGROUND: Inflammatory bowel disease, an autoimmune disease, has two clinical 
      manifestations including Crohn's disease and ulcerative colitis (UC). IL-17 has 
      been the target of intensive research in autoimmune diseases. The influence of 
      Toll like receptor 4 (TLR-4) gene polymorphisms on IL-17 production has also been 
      revealed in UC patients and tissue inflammation in mice. OBJECTIVES: To 
      investigate the association between the TLR-4 gene polymorphisms, Asp299Gly and 
      Thr399Ile and IL-17 serum levels with ulcerative colitis. Additionally, we aimed 
      to study modulation effects of forenamed gene polymorphisms on IL-17 serum levels 
      in UC patients and controls. METHODS: A total of 256 healthy controls and 85 UC 
      patients enrolled in our study. DNA was extracted and PCR-RFLP technique was 
      employed to determine Asp299Gly and Thr399Ile polymorphisms in TLR-4 gene and 
      IL-17 serum levels were measured by ELISA method. RESULTS: There was no 
      significant difference between the frequency of Asp299Gly A>G and Thr399Ile C>T 
      in UC patients and controls. While IL-17 serum levels in UC patients were 
      significantly higher than controls (p=0.003), no significant difference in IL-17 
      levels between different genotypes existed. Additionally, a significant inverse 
      relationship was observed between hemoglobin level and IL-17 serum levels in UC 
      patients (p=0.039). CONCLUSIONS: Increased IL-17 serum levels in our UC patients 
      might be explained through the synergistic activity of IL-17/IL-23 axis and 
      pro-inflammatory cytokines, causing severe clinical outcome in patients with IBD. 
      The prolonged excretion of blood in stool driven by inflammatory process which 
      causes iron metabolism disorder and anemia may elucidate the inverse correlation 
      between hemoglobin and IL-17 serum levels in UC patients. Lack of association 
      between the TLR-4 gene polymorphisms and UC in our study was consistent with the 
      results from other Caucasian populations.
FAU - Mohammadi, Mojgan
AU  - Mohammadi M
AD  - Physiology Research Centre, Kerman University of Medical Sciences, Kerman, Iran, 
      e-mail: mozhganmohammadi69@yahoo.co.uk.
FAU - Zahedi, Mohammad Javad
AU  - Zahedi MJ
FAU - Nikpoor, Amin Reza
AU  - Nikpoor AR
FAU - Baneshi, Mohammad Reza
AU  - Baneshi MR
FAU - Hayatbakhsh, Mohammad Mahdi
AU  - Hayatbakhsh MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Immunol
JT  - Iranian journal of immunology : IJI
JID - 101282932
RN  - 0 (Hemoglobins)
RN  - 0 (Interleukin-17)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Colitis, Ulcerative/genetics/*immunology
MH  - DNA Mutational Analysis
MH  - Female
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Hemoglobins/metabolism
MH  - Humans
MH  - Interleukin-17/*blood
MH  - Iran
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Toll-Like Receptor 4/*genetics
MH  - Young Adult
EDAT- 2013/07/03 06:00
MHDA- 2014/07/23 06:00
CRDT- 2013/07/02 06:00
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2014/07/23 06:00 [medline]
AID - 03 [pii]
PST - ppublish
SO  - Iran J Immunol. 2013 Jun;10(2):83-92.