PMID- 23812879
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131017
LR  - 20211021
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 405
IP  - 21
DP  - 2013 Aug
TI  - Off-chip passivated-electrode, insulator-based dielectrophoresis (OpiDEP).
PG  - 6657-66
LID - 10.1007/s00216-013-7123-7 [doi]
AB  - In this study, we report the first off-chip passivated-electrode, insulator-based 
      dielectrophoresis microchip (OpiDEP). This technique combines the sensitivity of 
      electrode-based dielectrophoresis (eDEP) with the high-throughput and inexpensive 
      device characteristics of insulator-based dielectrophoresis (iDEP). The device is 
      composed of a permanent, reusable set of electrodes and a disposable, polymer 
      microfluidic chip with microposts embedded in the microchannel. The device 
      operates by capacitively coupling the electric fields into the microchannel; 
      thus, no physical connections are made between the electrodes and the 
      microfluidic device. During operation, the polydimethylsiloxan (PDMS) 
      microfluidic chip fits onto the electrode substrate as a disposable cartridge. 
      OpiDEP uses insulting structures within the channel as well as parallel electrodes 
      to create DEP forces by the same working principle that iDEP devices use. The 
      resulting devices create DEP forces which are larger by two orders of magnitude 
      for the same applied voltage when compared to off-chip eDEP designs from 
      literature, which rely on parallel electrodes alone to produce the DEP forces. 
      The larger DEP forces allow the OpiDEP device to operate at high flow rates 
      exceeding 1 mL/h. In order to demonstrate this technology, Escherichia coli (E. 
      coli), a known waterborne pathogen, was trapped from water samples. Trapping 
      efficiencies of 100% were obtained at flow rates as high as 400 muL/h and 60% at 
      flow rates as high as 1200 muL/h. Additionally, bacteria were selectively 
      concentrated from a suspension of polystyrene beads.
FAU - Zellner, Phillip
AU  - Zellner P
AD  - VT MEMS Lab, Department of Electrical and Computer Engineering, Virginia Tech, 
      Blacksburg, VA 24060, USA.
FAU - Shake, Tyler
AU  - Shake T
FAU - Sahari, Ali
AU  - Sahari A
FAU - Behkam, Bahareh
AU  - Behkam B
FAU - Agah, Masoud
AU  - Agah M
LA  - eng
PT  - Journal Article
DEP - 20130630
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
PMC - PMC3730152
EDAT- 2013/07/03 06:00
MHDA- 2013/07/03 06:01
PMCR- 2013/06/30
CRDT- 2013/07/02 06:00
PHST- 2013/04/04 00:00 [received]
PHST- 2013/06/05 00:00 [accepted]
PHST- 2013/05/24 00:00 [revised]
PHST- 2013/07/02 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2013/07/03 06:01 [medline]
PHST- 2013/06/30 00:00 [pmc-release]
AID - 7123 [pii]
AID - 10.1007/s00216-013-7123-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2013 Aug;405(21):6657-66. doi: 10.1007/s00216-013-7123-7. Epub 
      2013 Jun 30.