PMID- 23816715
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20191112
IS  - 1319-2442 (Print)
IS  - 1319-2442 (Linking)
VI  - 24
IP  - 4
DP  - 2013 Jul
TI  - Tumor necrosis factor-alfa and monocyte chemoattractant protein-1 gene 
      polymorphisms in kidney transplant recipients.
PG  - 688-95
AB  - Tumor necrosis factor-alfa (TNF-alpha) gene polymorphism is supposed to have a 
      significant influence on the incidence of acute rejection in renal 
      transplantation. The monocyte chemoattractant protein-1 (MCP-1) is another factor 
      supposed to modulate graft rejection. We studied TNF-alpha and MCP-1 gene 
      polymorphisms in 84 kidney allograft recipients with polymerase chain reaction 
      and restriction fragment length polymorphism and their serum levels by 
      enzyme-linked immunosorbent assay. The patients were classified into two groups 
      based on their outcomes: Group I (n = 47) recipients with stable graft function 
      as the control group and group II (n=37) recipients who experienced acute graft 
      rejection episodes in the first 30 days post-transplantation. A significantly 
      higher incidence of TNF 2 /TNF 2 genotype was observed among patients with acute 
      graft rejection in comparison with the control group (40.5% and 19.2% 
      respectively, P <0.05), while no statistically significant differences were 
      observed in the TNF 1 /TNF 1 genotype between the groups (59.4% and 80.8%, 
      respectively, P >0.05). A significant elevation of serum TNF-alpha levels was found 
      in group II than group I and between TNF 2 genotype compared with that of TNF1 
      genotype within group II recipients. Distribution of MCP-1 genotypes in patients 
      with and without acute rejection episodes was not significantly different (70.2% 
      and 76.6% for MCP-1 A/A and 29.7% and 23.4% for MCP-1 G/G, respectively, P 
      >0.05). The serum MCP-1 levels were not significantly different between the 
      groups and between MCP-1 G/G genotype and MCP-1 A/A genotype in group II 
      recipients. In conclusion, TNF-alpha gene polymorphism or its serum levels may 
      identify patients at risk of acute rejection, where patients with TNF 2 /TNF 2 
      genotype or high serum TNF-alpha levels are more likely to have acute rejection 
      episodes, while there was no relation between MCP-1 genotype or its serum levels 
      and acute rejection.
FAU - El-Gezawy, Ebtesam M
AU  - El-Gezawy EM
AD  - Clinical Pathology Department, Assiut University, Assiut, Egypt.
FAU - Eldin, Eman Nasr
AU  - Eldin EN
FAU - Mohamed, Wafaa S
AU  - Mohamed WS
FAU - Mahmoud, Maged S
AU  - Mahmoud MS
FAU - Saied, Seham Ahmed
AU  - Saied SA
FAU - Abd El-Latif, Hanan Hareth
AU  - Abd El-Latif HH
FAU - Ibrahim, Maha Atwa
AU  - Ibrahim MA
LA  - eng
PT  - Journal Article
PL  - Saudi Arabia
TA  - Saudi J Kidney Dis Transpl
JT  - Saudi journal of kidney diseases and transplantation : an official publication of 
      the Saudi Center for Organ Transplantation, Saudi Arabia
JID - 9436968
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Chemokine CCL2/*genetics
MH  - Female
MH  - Humans
MH  - *Kidney Transplantation
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2013/07/03 06:00
MHDA- 2013/09/04 06:00
CRDT- 2013/07/03 06:00
PHST- 2013/07/03 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - SaudiJKidneyDisTranspl_2013_24_4_688_113855 [pii]
AID - 10.4103/1319-2442.113855 [doi]
PST - ppublish
SO  - Saudi J Kidney Dis Transpl. 2013 Jul;24(4):688-95. doi: 10.4103/1319-2442.113855.