PMID- 23816956
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 31
IP  - 6
DP  - 2013
TI  - Oxidized low-density lipoprotein induces inflammatory responses in cultured human 
      mast cells via Toll-like receptor 4.
PG  - 842-53
LID - 10.1159/000350102 [doi]
AB  - BACKGROUND/AIMS: Oxidized low-density lipoprotein (ox-LDL) is a powerful 
      atherogen. Toll-like receptor 4 (TLR4) has a pathophysiological role in 
      regulating inflammatory responses and atherosclerosis. Mast cells can infiltrate 
      into the atheromatous plaque and secrete various pro-inflammatory cytokines, 
      which significantly amplify the atherogenic processes and promote plaque 
      vulnerability. Small interfering RNA (siRNA) is an effective method to silence 
      the target genes. We evaluated whether ox-LDL-induced inflammation depended in 
      part on the activation of TLR4-dependent signaling pathways in a cultured human 
      mast cell line (HMC-1). METHOD: HMC-1 cells were cultured, and treated with 
      ox-LDL, TLR4-specific siRNA, or inhibitors of phosphorylation of 
      mitogen-activated protein kinase (MAPKs), and nuclear factor-kappaB (NF-kappaB), a 
      critical mediator of inflammation. The expression of monocyte chemoattractant 
      protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6) was 
      measured subsequently. RESULTS: Ox-LDL increased the expression of TLR4 and 
      secretion of MCP-1, TNF-alpha and IL-6. Moreover, ox-LDL stimulated the translocation 
      of NF-kappaB, from the cytoplasm to nucleus. Additionally, phosphorylation of MAPK 
      was greatly increased. These ox-LDL-induced alterations were significantly 
      attenuated by pretreatment with TLR4-specific siRNA. CONCLUSION: Ox-LDL induced 
      inflammatory responses in cultured HMC-1 cells including NF-kappaB nuclear 
      translocation and phosphorylation of MAPKs, a process mediated in part by TLR4.
CI  - Copyright (c) 2013 S. Karger AG, Basel.
FAU - Meng, Zhe
AU  - Meng Z
AD  - Department of Cardiology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University School of Medicine, Xi'an, Shaanxi, China. mengzhenihao@163.com
FAU - Yan, Chao
AU  - Yan C
FAU - Deng, Qian
AU  - Deng Q
FAU - Dong, Xin
AU  - Dong X
FAU - Duan, Zong-Ming
AU  - Duan ZM
FAU - Gao, Deng-Feng
AU  - Gao DF
FAU - Niu, Xiao-Lin
AU  - Niu XL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130607
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Chemokine CCL2)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (oxidized low density lipoprotein)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Humans
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Lipoproteins, LDL/*pharmacology
MH  - Mast Cells/cytology/*drug effects/metabolism
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Phosphorylation/drug effects
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Toll-Like Receptor 4/antagonists & inhibitors/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2013/07/03 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/07/03 06:00
PHST- 2013/05/14 00:00 [accepted]
PHST- 2013/07/03 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - 000350102 [pii]
AID - 10.1159/000350102 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2013;31(6):842-53. doi: 10.1159/000350102. Epub 2013 Jun 7.