PMID- 23818296
OWN - NLM
STAT- MEDLINE
DCOM- 20140428
LR  - 20181202
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 124
IP  - 3
DP  - 2014 Mar
TI  - Improved wound healing of postischemic cutaneous flaps with the use of bone 
      marrow-derived stem cells.
PG  - 642-8
LID - 10.1002/lary.24293 [doi]
AB  - OBJECTIVES/HYPOTHESIS: To determine if the intravascular delivery of mesenchymal 
      stem cells improves wound healing and blood perfusion to postischemic cutaneous 
      flap tissues. STUDY DESIGN: Randomized controlled study. METHODS: A murine model 
      of a cutaneous flap was created based on the inferior epigastric vessels. Mice 
      (n = 14) underwent 3.5 hours of ischemia followed by reperfusion. Bone marrow 
      stromal cells (BMSCs) 1 x 10(6) were injected intravenously. Wound healing was 
      then assessed measuring percent flap necrosis, flap perfusion, and tensile 
      strength of the flap after a period of 14 days. Localization of BMSCs was 
      determined with radiolabeled and fluorescent labeled BMSCs. RESULTS: Postischemic 
      cutaneous flap tissues treated with BMSCs demonstrated significantly less 
      necrosis than control flaps (P <0.01). Beginning on postoperative day 5, 
      BMSC-treated flaps demonstrated greater blood perfusion than untreated flaps (P 
      <0.01). Tensile strength of BMSC-treated cutaneous flaps was significantly higher 
      (P <0.01), with a mean strength of 283.4 +/- 28.4 N/m than control flaps with a 
      mean of 122.4 +/- 23.5 N/m. Radiolabeled BMSCs localized to postischemic flaps 
      compared to untreated tissues (P = 0.001). Fluorescent microscopy revealed 
      incorporation of BMSCs into endothelial and epithelial tissues of postischemic 
      flaps. CONCLUSIONS: This study demonstrates that the intravascular delivery of 
      BMSCs increases wound healing and promotes flap survival following 
      ischemia-reperfusion injury of cutaneous tissue flaps.
CI  - (c) 2013 The American Laryngological, Rhinological and Otological Society, Inc.
FAU - Hu, Melissa
AU  - Hu M
AD  - Department of Otolaryngology-Head and Neck Surgery, LSUH-S, Shreveport, 
      Louisiana, U.S.A.
FAU - Ludlow, David
AU  - Ludlow D
FAU - Alexander, J Steven
AU  - Alexander JS
FAU - McLarty, Jerry
AU  - McLarty J
FAU - Lian, Timothy
AU  - Lian T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130719
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
SB  - IM
EIN - Laryngoscope. 2013 Dec;123(12):3243
MH  - Animals
MH  - Cell Movement/physiology
MH  - Disease Models, Animal
MH  - Graft Survival
MH  - Infusions, Intravenous
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Necrosis/pathology
MH  - Random Allocation
MH  - Regional Blood Flow/physiology
MH  - Reperfusion Injury/pathology/*therapy
MH  - Risk Assessment
MH  - Statistics, Nonparametric
MH  - Surgical Flaps/blood supply/*pathology
MH  - Tensile Strength
MH  - Treatment Outcome
MH  - Wound Healing/*physiology
OTO - NOTNLM
OT  - Bone marrow stromal cells
OT  - Level of Evidence: NA
OT  - cutaneous flaps
OT  - free tissue transfer
OT  - ischemia
OT  - stem cells
OT  - wound healing
EDAT- 2013/07/03 06:00
MHDA- 2014/04/29 06:00
CRDT- 2013/07/03 06:00
PHST- 2013/03/27 00:00 [received]
PHST- 2013/06/02 00:00 [revised]
PHST- 2013/06/13 00:00 [accepted]
PHST- 2013/07/03 06:00 [entrez]
PHST- 2013/07/03 06:00 [pubmed]
PHST- 2014/04/29 06:00 [medline]
AID - 10.1002/lary.24293 [doi]
PST - ppublish
SO  - Laryngoscope. 2014 Mar;124(3):642-8. doi: 10.1002/lary.24293. Epub 2013 Jul 19.