PMID- 23820116
OWN - NLM
STAT- MEDLINE
DCOM- 20140403
LR  - 20220408
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 155
IP  - 1-2
DP  - 2013 Sep 1
TI  - Disparate effects of LPS infusion and carbohydrate overload on inflammatory gene 
      expression in equine laminae.
PG  - 1-8
LID - S0165-2427(13)00146-3 [pii]
LID - 10.1016/j.vetimm.2013.05.001 [doi]
AB  - Although clinical evidence of endotoxemia has been associated with the 
      development of acute laminitis in hospitalized horses with gastrointestinal 
      diseases and endotoxins have been detected in the circulation of horses with 
      experimentally-induced laminitis, it is unclear what role, if any, endotoxins 
      have play the pathogenesis of the disease. Therefore, in the present study we 
      compared the effects of endotoxin infusion to that of intra-gastric 
      administration of mixed carbohydrate (CHO) on clinical signs of laminitis, plasma 
      concentrations of TNF-alpha and IL-10, and laminar tissue expression of 20 genes 
      associated with inflammation. Horses were divided into 4 groups: Control (water 
      placebo, n=7), endotoxin infusion (LPS, n=6), CHO/Developmental (30% decrease in 
      central venous pressure, n=6) and CHO/Lame (Obel grade I laminitis, n=7). Horses 
      in the LPS group developed clinical signs consistent with systemic inflammation, 
      had rapid increases in plasma concentrations of both TNF-alpha and IL-10, and 
      leukopenia, but did not have any changes in laminar tissue expression of the 
      genes associated with inflammation. In contrast, horses administered CHO 
      developed clinical signs consistent with systemic inflammation, had more delayed 
      increases in TNF-alpha, IL-10 and total leukocyte counts, and had marked increases in 
      laminar tissue expression of the genes associated with inflammation. Only the 
      horses administered CHO developed clinical signs of laminitis, providing 
      additional credence to the concept that factors other than endotoxin are 
      responsible for the changes in laminar tissue gene expression that occur during 
      the development of acute equine laminitis.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Kwon, S
AU  - Kwon S
AD  - Department of Large Animal Medicine, College of Veterinary Medicine, University 
      of Georgia, Athens, GA 30602, USA.
FAU - Moore, J N
AU  - Moore JN
FAU - Robertson, T P
AU  - Robertson TP
FAU - Hurley, D J
AU  - Hurley DJ
FAU - Wagner, B
AU  - Wagner B
FAU - Vandenplas, M L
AU  - Vandenplas ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Dietary Carbohydrates/administration & dosage
MH  - Endotoxemia/genetics/immunology/veterinary
MH  - Female
MH  - Foot Diseases/genetics/immunology/*veterinary
MH  - Gene Expression/drug effects
MH  - *Hoof and Claw
MH  - Horse Diseases/*etiology/genetics/immunology
MH  - Horses/*genetics/*immunology
MH  - Inflammation/genetics/immunology/*veterinary
MH  - Interleukin-10/blood
MH  - Lameness, Animal/etiology/genetics/immunology
MH  - Lipopolysaccharides/administration & dosage
MH  - Male
MH  - Tumor Necrosis Factor-alpha/blood
OTO - NOTNLM
OT  - Carbohydrate
OT  - Endotoxin
OT  - Equine
OT  - Gene expression
OT  - Laminitis
OT  - Lipopolysaccharide
EDAT- 2013/07/04 06:00
MHDA- 2014/04/04 06:00
CRDT- 2013/07/04 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/04/24 00:00 [revised]
PHST- 2013/05/01 00:00 [accepted]
PHST- 2013/07/04 06:00 [entrez]
PHST- 2013/07/04 06:00 [pubmed]
PHST- 2014/04/04 06:00 [medline]
AID - S0165-2427(13)00146-3 [pii]
AID - 10.1016/j.vetimm.2013.05.001 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2013 Sep 1;155(1-2):1-8. doi: 
      10.1016/j.vetimm.2013.05.001. Epub 2013 Jun 10.