PMID- 23822128
OWN - NLM
STAT- MEDLINE
DCOM- 20140130
LR  - 20130704
IS  - 1757-6199 (Electronic)
IS  - 1757-6180 (Linking)
VI  - 5
IP  - 13
DP  - 2013 Jul
TI  - Automating bioanalytical sample analysis through enhanced system integration.
PG  - 1649-59
LID - 10.4155/bio.13.123 [doi]
AB  - BACKGROUND: In the bioanalytical laboratory, challenges associated with manual, 
      repetitive, labor-intensive processes can be addressed by powerful automated 
      liquid handlers; however, their implementation has been difficult due to lack of 
      efficient integration into laboratory workflows. Faster throughput is afforded to 
      ligand binding assay (LBA) technologies via enhanced automation, but the upstream 
      sample processing still remains a bottleneck. To be truly efficient, these 
      technologies must be incorporated into a laboratory information management system 
      (LIMS) to streamline data analysis and reporting. RESULTS: Three off-the-shelf 
      technologies that aid in improving bioanalytical laboratory efficiencies were 
      utilized with minimal customization to streamline the sample analysis process. 
      Information extracted via a sequence file from the LIMS run was utilized to 
      perform the sample processing on the automated liquid handler. A file conversion 
      tool converted the sequence files that allowed for sample processing and 
      preparing the assay ready plate. The plate was then transferred to the LBA 
      microfluidics platform to run the experiments. The integration was tested using a 
      LBA PK assay that demonstrated good sample dilution and assay performance. 
      CONCLUSION: We successfully integrated LIMS with an automated liquid handler and 
      a microfluidics platform to automate the sample analysis process in the 
      bioanalytical laboratory. The utilization of off-the-shelf technologies with 
      minimal customization requires minimal resources from laboratory scientists. It 
      may be possible to implement this approach for other analytical platforms.
FAU - Patel, Vimal
AU  - Patel V
AD  - Amgen Inc., Amgen Center Drive, Thousand Oaks, CA 91320-1799, USA. 
      vimalp@amgen.com
FAU - Leach, Daniel
AU  - Leach D
FAU - Hornberger, Mark
AU  - Hornberger M
FAU - Williams, Kathi
AU  - Williams K
FAU - Shih, Judy
AU  - Shih J
FAU - Ma, Mark
AU  - Ma M
FAU - Laycock, John
AU  - Laycock J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Bioanalysis
JT  - Bioanalysis
JID - 101512484
SB  - IM
MH  - Chemistry Techniques, Analytical/*methods/standards
MH  - Informatics/*methods
MH  - Laboratories
MH  - Quality Control
MH  - Reproducibility of Results
EDAT- 2013/07/05 06:00
MHDA- 2014/01/31 06:00
CRDT- 2013/07/05 06:00
PHST- 2013/07/05 06:00 [entrez]
PHST- 2013/07/05 06:00 [pubmed]
PHST- 2014/01/31 06:00 [medline]
AID - 10.4155/bio.13.123 [doi]
PST - ppublish
SO  - Bioanalysis. 2013 Jul;5(13):1649-59. doi: 10.4155/bio.13.123.