PMID- 23824804
OWN - NLM
STAT- MEDLINE
DCOM- 20131106
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 18
DP  - 2013 Sep
TI  - Evolutionarily conserved epitopes on human immunodeficiency virus type 1 (HIV-1) 
      and feline immunodeficiency virus reverse transcriptases detected by 
      HIV-1-infected subjects.
PG  - 10004-15
LID - 10.1128/JVI.00359-13 [doi]
AB  - Anti-human immunodeficiency virus (HIV) cytotoxic T lymphocyte (CTL)-associated 
      epitopes, evolutionarily conserved on both HIV type 1 (HIV-1) and feline 
      immunodeficiency virus (FIV) reverse transcriptases (RT), were identified using 
      gamma interferon (IFN-gamma) enzyme-linked immunosorbent spot (ELISpot) and 
      carboxyfluorescein diacetate succinimide ester (CFSE) proliferation assays 
      followed by CTL-associated cytotoxin analysis. The peripheral blood mononuclear 
      cells (PBMC) or T cells from HIV-1-seropositive (HIV(+)) subjects were stimulated 
      with overlapping RT peptide pools. The PBMC from the HIV(+) subjects had more 
      robust IFN-gamma responses to the HIV-1 peptide pools than to the FIV peptide pools, 
      except for peptide-pool F3. In contrast, much higher and more frequent CD8(+) 
      T-cell proliferation responses were observed with the FIV peptide pools than with 
      the HIV peptide pools. HIV-1-seronegative subjects had no proliferation or IFN-gamma 
      responses to the HIV and FIV peptide pools. A total of 24% (40 of 166) of the 
      IFN-gamma responses to HIV pools and 43% (23 of 53) of the CD8(+) T-cell 
      proliferation responses also correlated to responses to their counterpart FIV 
      pools. Thus, more evolutionarily conserved functional epitopes were identified by 
      T-cell proliferation than by IFN-gamma responses. In the HIV(+) subjects, 
      peptide-pool F3, but not the HIV H3 counterpart, induced the most IFN-gamma and 
      proliferation responses. These reactions to peptide-pool F3 were highly 
      reproducible and persisted over the 1 to 2 years of testing. All five individual 
      peptides and epitopes of peptide-pool F3 induced IFN-gamma and/or proliferation 
      responses in addition to inducing CTL-associated cytotoxin responses (perforin, 
      granzyme A, granzyme B). The epitopes inducing polyfunctional T-cell activities 
      were highly conserved among human, simian, feline, and ungulate lentiviruses, 
      which indicated that these epitopes are evolutionarily conserved. These results 
      suggest that FIV peptides could be used in an HIV-1 vaccine.
FAU - Sanou, Missa P
AU  - Sanou MP
AD  - Department of Infectious Diseases and Pathology, College of Veterinary Medicine, 
      University of Florida, Gainesville, Florida, USA.
FAU - Roff, Shannon R
AU  - Roff SR
FAU - Mennella, Antony
AU  - Mennella A
FAU - Sleasman, John W
AU  - Sleasman JW
FAU - Rathore, Mobeen H
AU  - Rathore MH
FAU - Yamamoto, Janet K
AU  - Yamamoto JK
FAU - Levy, Jay A
AU  - Levy JA
LA  - eng
GR  - UL1 RR029890/RR/NCRR NIH HHS/United States
GR  - R01 AI030904/AI/NIAID NIH HHS/United States
GR  - P30 AI027763/AI/NIAID NIH HHS/United States
GR  - R01 AI065276/AI/NIAID NIH HHS/United States
GR  - R01-AI30904/AI/NIAID NIH HHS/United States
GR  - R01-AI65276/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130703
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Epitopes)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cell Proliferation
MH  - Conserved Sequence
MH  - Enzyme-Linked Immunospot Assay
MH  - Epitopes/genetics/*immunology
MH  - Female
MH  - HIV-1/genetics/*immunology
MH  - Humans
MH  - Immunodeficiency Virus, Feline/genetics/*immunology
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Middle Aged
MH  - RNA-Directed DNA Polymerase/genetics/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Young Adult
PMC - PMC3754003
EDAT- 2013/07/05 06:00
MHDA- 2013/11/07 06:00
PMCR- 2014/03/01
CRDT- 2013/07/05 06:00
PHST- 2013/07/05 06:00 [entrez]
PHST- 2013/07/05 06:00 [pubmed]
PHST- 2013/11/07 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - JVI.00359-13 [pii]
AID - 00359-13 [pii]
AID - 10.1128/JVI.00359-13 [doi]
PST - ppublish
SO  - J Virol. 2013 Sep;87(18):10004-15. doi: 10.1128/JVI.00359-13. Epub 2013 Jul 3.