PMID- 23825146
OWN - NLM
STAT- MEDLINE
DCOM- 20140205
LR  - 20211021
IS  - 1862-1783 (Electronic)
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 14
IP  - 7
DP  - 2013 Jul
TI  - Identification and determination of major constituents in a traditional Chinese 
      medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MS(n) and 
      HPLC-UV/ELSD.
PG  - 604-14
LID - 10.1631/jzus.B1200295 [doi]
AB  - Xiongdankaiming tablet (XDKMT), a well-known compound in traditional Chinese 
      medicine, is widely used for the treatment of acute iridocyclitis and primary 
      open-angle glaucoma. In this paper, accurate and reliable methods were developed 
      for the identification of 20 constituents using high-performance liquid 
      chromatography with photo-diode array and electron spray ionization-mass 
      spectrometry (HPLC-PDA/ESI-MS(n)), and determination of nine of the constituents 
      (chlorogenic acid, gentiopicroside, isochlorogenic acid B, 
      diosmetin-7-O-beta-d-glucopyranoside, apigenin, diosmetin, tauroursodeoxycholic 
      acid, acacetin, and taurochenodeoxycholic acid) was developed using HPLC with 
      ultraviolet absorption detector and evaporative light scattering detector 
      (HPLC-UV/ELSD) for the first time. The best results were obtained on a Zorbax 
      SB-C18 column with gradient elution using water (0.1% formic acid) (A) and 
      methanol (0.1% formic acid) (B) at a  fl ow rate of 0.7 ml/min. Tauroursodeoxycholic 
      acid and taurochenodeoxycholic acid, owing to their low UV absorption, were 
      detected by ELSD. The other seven compounds were analyzed by HPLC-UV with 
      variable wavelengths. The calibration curves of all nine constituents showed good 
      linear regression (R(2)>0.9996) within the linearity ranges. The limits of 
      detection and quantification were in the ranges of 0.0460-9.90 mug/ml and 
      0.115-24.8 mug/ml, respectively. The accuracy, in terms of recovery, varied from 
      95.3% to 104.9% with relative standard deviations (RSDs) less than 4.4%. 
      Precision (with the intra- and inter-day variations less than 4.4%) was also 
      suitable for its intended use. The developed method was successfully applied for 
      the analysis of major components in XDKMT, which provides an appropriate method 
      for the quality control of XDKMT.
FAU - Wang, Shu-Fang
AU  - Wang SF
AD  - Institute of Pharmaceutical Informatics, Zhejiang University, Hangzhou 310058, 
      China. wangsf@zju.edu.cn
FAU - Leng, Jing
AU  - Leng J
FAU - Xu, Yi-Min
AU  - Xu YM
FAU - Feng, Mei-Ling
AU  - Feng ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Tablets)
RN  - 0 (Xiongdankaiming)
SB  - IM
MH  - Calibration
MH  - Chemistry, Pharmaceutical/methods
MH  - Chromatography/methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*analysis/chemistry
MH  - Light
MH  - Medicine, Chinese Traditional
MH  - Quality Control
MH  - Regression Analysis
MH  - Reproducibility of Results
MH  - Scattering, Radiation
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tablets
MH  - Ultraviolet Rays
PMC - PMC3709065
COIS- Compliance with ethics guidelines: Shu-fang WANG, Jing LENG, Yi-min XU, and 
      Mei-ling FENG declare that they have no conflict of interest. This article does 
      not contain any studies with human or animal subjects performed by any of the 
      authors.
EDAT- 2013/07/05 06:00
MHDA- 2014/02/06 06:00
PMCR- 2013/07/01
CRDT- 2013/07/05 06:00
PHST- 2013/07/05 06:00 [entrez]
PHST- 2013/07/05 06:00 [pubmed]
PHST- 2014/02/06 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - 10.1631/jzus.B1200295 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2013 Jul;14(7):604-14. doi: 10.1631/jzus.B1200295.