PMID- 23825458
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130705
LR  - 20211021
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 4
DP  - 2013
TI  - The oligodendroglial precursor cell line Oli-neu represents a cell culture system 
      to examine functional expression of the mouse gap junction gene connexin29 
      (Cx29).
PG  - 83
LID - 10.3389/fphar.2013.00083 [doi]
LID - 83
AB  - The potential gap junction forming mouse connexin29 (Cx29) protein is 
      concomitantly expressed with connexin32 (Cx32) in peripheral myelin forming 
      Schwann cells and together with both Cx32 and connexin47 (Cx47) in 
      oligodendrocytes of the CNS. To study the genomic structure and functional 
      expression of Cx29, either primary cells or cell culture systems might be 
      selected, from which the latter are easier to cultivate. Both structure and 
      expression of Cx29 is still not fully understood. In the mouse sciatic nerve, 
      brain and the oligodendroglial precursor cell line Oli-neu the Cx29 gene is 
      processed in two transcript isoforms both harboring a unique reading frame. In 
      contrast to Cx32 and Cx47, only Cx29 protein is abundantly expressed in 
      undifferentiated as well as differentiated Oli-neu cells but the absence of Etbr 
      dye transfer after microinjection concealed the function of Cx29-mediated gap 
      junction communication between those cells. Although HeLa cells stably 
      transfected with Cx29 or Cx29-eGFP neither demonstrated any permeability for 
      Lucifer yellow nor for neurobiotin, blocking of Etbr uptake from the media by gap 
      junction blockers does suppose a role of Cx29 in hemi-channel function. Thus, we 
      conclude that, due to its high abundance of Cx29 expression and its reproducible 
      culture conditions, the oligodendroglial precursor cell line Oli-neu might 
      constitute an appropriate cell culture system to study molecular mechanisms or 
      putative extracellular stimuli to functionally open Cx29 channels or 
      hemi-channels.
FAU - Sohl, Goran
AU  - Sohl G
AD  - Abteilung Molekulargenetik, Institut fur Genetik, Universitat Bonn Bonn, Germany.
FAU - Hombach, Sonja
AU  - Hombach S
FAU - Degen, Joachim
AU  - Degen J
FAU - Odermatt, Benjamin
AU  - Odermatt B
LA  - eng
PT  - Journal Article
DEP - 20130628
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC3695394
OTO - NOTNLM
OT  - Cx29
OT  - HeLa cells
OT  - connexin
OT  - dye transfer
OT  - gap junction
OT  - oligodendroglial precursor cell-line Oli-neu
EDAT- 2013/07/05 06:00
MHDA- 2013/07/05 06:01
PMCR- 2013/06/28
CRDT- 2013/07/05 06:00
PHST- 2013/02/14 00:00 [received]
PHST- 2013/06/10 00:00 [accepted]
PHST- 2013/07/05 06:00 [entrez]
PHST- 2013/07/05 06:00 [pubmed]
PHST- 2013/07/05 06:01 [medline]
PHST- 2013/06/28 00:00 [pmc-release]
AID - 10.3389/fphar.2013.00083 [doi]
PST - epublish
SO  - Front Pharmacol. 2013 Jun 28;4:83. doi: 10.3389/fphar.2013.00083. Print 2013.