PMID- 23825634
OWN - NLM
STAT- MEDLINE
DCOM- 20140207
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 6
DP  - 2013
TI  - Identification of a functionally distinct truncated BDNF mRNA splice variant and 
      protein in Trachemys scripta elegans.
PG  - e67141
LID - 10.1371/journal.pone.0067141 [doi]
LID - e67141
AB  - Brain-derived neurotrophic factor (BDNF) has a diverse functional role and 
      complex pattern of gene expression. Alternative splicing of mRNA transcripts 
      leads to further diversity of mRNAs and protein isoforms. Here, we describe the 
      regulation of BDNF mRNA transcripts in an in vitro model of eyeblink classical 
      conditioning and a unique transcript that forms a functionally distinct truncated 
      BDNF protein isoform. Nine different mRNA transcripts from the BDNF gene of the 
      pond turtle Trachemys scripta elegans (tBDNF) are selectively regulated during 
      classical conditioning: exon I mRNA transcripts show no change, exon II 
      transcripts are downregulated, while exon III transcripts are upregulated. One 
      unique transcript that codes from exon II, tBDNF2a, contains a 40 base pair 
      deletion in the protein coding exon that generates a truncated tBDNF protein. The 
      truncated transcript and protein are expressed in the naive untrained state and 
      are fully repressed during conditioning when full-length mature tBDNF is 
      expressed, thereby having an alternate pattern of expression in conditioning. 
      Truncated BDNF is not restricted to turtles as a truncated mRNA splice variant 
      has been described for the human BDNF gene. Further studies are required to 
      determine the ubiquity of truncated BDNF alternative splice variants across 
      species and the mechanisms of regulation and function of this newly recognized 
      BDNF protein.
FAU - Ambigapathy, Ganesh
AU  - Ambigapathy G
AD  - Neuroscience Group, Division of Basic Biomedical Sciences, University of South 
      Dakota Sanford School of Medicine, Vermillion, South Dakota, United States of 
      America.
FAU - Zheng, Zhaoqing
AU  - Zheng Z
FAU - Li, Wei
AU  - Li W
FAU - Keifer, Joyce
AU  - Keifer J
LA  - eng
GR  - R01 NS051187/NS/NINDS NIH HHS/United States
GR  - NS051187/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130625
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reptilian Proteins)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/chemistry/*genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neurons/cytology
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Reptilian Proteins/chemistry/*genetics/*metabolism
MH  - *Sequence Deletion
MH  - Signal Transduction
MH  - *Turtles
PMC - PMC3692439
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/07/05 06:00
MHDA- 2014/02/08 06:00
PMCR- 2013/06/25
CRDT- 2013/07/05 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/05/14 00:00 [accepted]
PHST- 2013/07/05 06:00 [entrez]
PHST- 2013/07/05 06:00 [pubmed]
PHST- 2014/02/08 06:00 [medline]
PHST- 2013/06/25 00:00 [pmc-release]
AID - PONE-D-13-03340 [pii]
AID - 10.1371/journal.pone.0067141 [doi]
PST - epublish
SO  - PLoS One. 2013 Jun 25;8(6):e67141. doi: 10.1371/journal.pone.0067141. Print 2013.