PMID- 23826954
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20190318
IS  - 1480-3275 (Electronic)
IS  - 0008-4166 (Linking)
VI  - 59
IP  - 7
DP  - 2013 Jul
TI  - Predominance of ammonia-oxidizing archaea and nirK-gene-bearing denitrifiers 
      among ammonia-oxidizing and denitrifying populations in sediments of a large 
      urban eutrophic lake (Lake Donghu).
PG  - 456-64
LID - 10.1139/cjm-2013-0083 [doi]
AB  - The coupled nitrification-denitrification process plays a pivotal role in cycling 
      and removal of nitrogen in aquatic ecosystems. In the present study, the 
      communities of ammonia oxidizers and denitrifiers in the sediments of 2 basins 
      (Guozhenghu Basin and Tuanhu Basin) of a large urban eutrophic lake (Lake Donghu) 
      were determined using the ammonia monooxygenase subunit A (amoA) gene and the 
      nitrite reductase gene. At all sites of this study, the archaeal amoA gene 
      predominated over the bacterial amoA gene, whereas the functional gene for 
      denitrification nirK gene far outnumbered the nirS gene. Spatially, compared with 
      the Tuanhu Basin, the Guozhenghu Basin showed a significantly greater abundance 
      of the archaeal amoA gene but less abundance of the nirK and nirS genes, while 
      there was no significant difference of bacterial amoA gene copy numbers between 
      the 2 basins. Unlike the archaeal amoA gene, the nirK gene showed a significant 
      difference in community structure between the 2 basins. Archaeal amoA diversity 
      was limited to the water-sediment cluster of Crenarchaeota, in sharp contrast 
      with nirK for which 22 distinct operational taxonomic units were found. 
      Accumulation of organic substances were found to be positively related to nirK 
      and nirS gene copy numbers but negatively related to archaeal amoA gene copy 
      numbers, whereas the abundance of the bacterial amoA gene was related to ammonia 
      concentration.
FAU - Hou, Jie
AU  - Hou J
AD  - The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of 
      Hydrobiology, the Chinese Academy of Sciences, No. 7 Donghu South Road, Wuchang 
      District, Wuhan, Hubei, People's Republic of China.
FAU - Cao, Xiuyun
AU  - Cao X
FAU - Song, Chunlei
AU  - Song C
FAU - Zhou, Yiyong
AU  - Zhou Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130509
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.- (Nitrite Reductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/*classification/genetics/isolation & purification/metabolism
MH  - Bacteria/*classification/genetics/isolation & purification/metabolism
MH  - Biodiversity
MH  - Cities
MH  - Crenarchaeota/genetics/isolation & purification/metabolism
MH  - Denitrification
MH  - *Eutrophication
MH  - Genes, Archaeal
MH  - Genes, Bacterial
MH  - Geologic Sediments/microbiology
MH  - Lakes/*microbiology
MH  - Nitrite Reductases/genetics
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
EDAT- 2013/07/06 06:00
MHDA- 2013/09/17 06:00
CRDT- 2013/07/06 06:00
PHST- 2013/07/06 06:00 [entrez]
PHST- 2013/07/06 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
AID - 10.1139/cjm-2013-0083 [doi]
PST - ppublish
SO  - Can J Microbiol. 2013 Jul;59(7):456-64. doi: 10.1139/cjm-2013-0083. Epub 2013 May 
      9.