PMID- 23828143
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20211021
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 58
IP  - 12
DP  - 2013 Dec
TI  - Enemas with N-acetylcysteine can reduce the level of oxidative damage in cells of 
      the colonic mucosa diverted from the faecal stream.
PG  - 3452-9
LID - 10.1007/s10620-013-2768-9 [doi]
AB  - BACKGROUND: Oxidative stress has been related to inflammation of the colonic 
      mucosa in patients with diversion colitis (DC). AIM: The purpose of this study 
      was to evaluate the antioxidants effects of n-acetylcysteine (NAC) in colon 
      segments without faecal stream. METHODS: Thirty-six Wistar rats were subjected to 
      diversion of the faecal stream by proximal colostomy and a distal mucosal colon 
      fistula. They were distributed into three experimental groups of 12 animals each; 
      the animals in each group underwent daily enemas containing saline solution 
      (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each 
      group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was 
      determined by histopathological analysis and stratified by inflammatory grading 
      scale. Oxidative DNA damage was measured by comet assay. The Mann-Whitney test 
      and ANOVA were used for statistical analysis; p<0.05 was considered significant. 
      RESULTS: The oxidative DNA damage in colon segments without faecal stream was 
      significantly lower in animals treated with either concentration of NAC than in 
      control group, regardless of the duration of intervention (p<0.01). CONCLUSIONS: 
      Intrarectal application of NAC reduces the inflammation as well as DNA oxidative 
      damage and could be beneficial as a complementary agent in the treatment of DC.
FAU - Martinez, Carlos Augusto Real
AU  - Martinez CA
AD  - Post-Graduate Program in Health Science, Sao Francisco University, Rua Rui 
      Barbosa, 255 apto. 32, Vila Boa Vista, Santo Andre, Sao Paulo, CEP: 09190-370, 
      Brazil, caomartinez@uol.com.br.
FAU - de Almeida, Marcos Goncalves
AU  - de Almeida MG
FAU - da Silva, Camila Moraes Goncalves
AU  - da Silva CM
FAU - Ribeiro, Marcelo Lima
AU  - Ribeiro ML
FAU - da Cunha, Fernando Lorenzetti
AU  - da Cunha FL
FAU - Rodrigues, Murilo Rocha
AU  - Rodrigues MR
FAU - Sato, Daniela Tiemi
AU  - Sato DT
FAU - Pereira, Jose Aires
AU  - Pereira JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130705
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Free Radical Scavengers)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
CIN - Dig Dis Sci. 2013 Dec;58(12):3386-8. PMID: 23979440
MH  - Acetylcysteine/*administration & dosage
MH  - Animals
MH  - Colectomy/adverse effects
MH  - Colitis/*drug therapy/etiology/metabolism
MH  - Colon/pathology
MH  - DNA Damage/drug effects
MH  - *Enema
MH  - Free Radical Scavengers/*administration & dosage
MH  - Intestinal Mucosa/drug effects/metabolism/pathology
MH  - Oxidative Stress/*drug effects
MH  - Pouchitis/*drug therapy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
EDAT- 2013/07/06 06:00
MHDA- 2014/01/22 06:00
CRDT- 2013/07/06 06:00
PHST- 2012/09/01 00:00 [received]
PHST- 2013/06/16 00:00 [accepted]
PHST- 2013/07/06 06:00 [entrez]
PHST- 2013/07/06 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
AID - 10.1007/s10620-013-2768-9 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2013 Dec;58(12):3452-9. doi: 10.1007/s10620-013-2768-9. Epub 2013 
      Jul 5.